Lane D A, Rampling M W, Kakkar V V
Clin Chim Acta. 1976 Aug 16;71(1):15-9. doi: 10.1016/0009-8981(76)90269-2.
A thrombin method for the determination of fibrinogen concentration has been compared with a non-enzymatic sulphite precipitation method on plasma from patients undergoing thrombolytic therapy with an intermittent plasminogen/streptokinase regime. There was very good agreement between the methods even when the determinations were made in the presence of high levels of fibrinogen degradation products (FDP), provided that the thrombin clottable fibrinogen was greater than 1 mg/ml. However, after extensive fibrinogen depletion, when the thrombin method underestimates fibrinogen concentration due to significant clot inhibition by FDP, an overestimate due to co-precipitation of fragments X and Y will be obtained by the sulphite method. This suggests that the simultaneous application of both techniques may provide a simple method of assessing the presence of high levels of anticoagulant FDP in various clinical disorders.
已将一种用于测定纤维蛋白原浓度的凝血酶法与一种非酶亚硫酸盐沉淀法，针对接受间歇性纤溶酶原/链激酶治疗方案的溶栓治疗患者的血浆进行了比较。即使在存在高水平纤维蛋白原降解产物（FDP）的情况下进行测定，只要凝血酶可凝固的纤维蛋白原大于1 mg/ml，两种方法之间也具有很好的一致性。然而，在纤维蛋白原大量消耗后，当凝血酶法由于FDP对凝块的显著抑制而低估纤维蛋白原浓度时，亚硫酸盐法将因片段X和Y的共沉淀而得到高估结果。这表明同时应用这两种技术可能提供一种简单的方法，用于评估各种临床病症中抗凝FDP高水平的存在情况。
