Kjeld J M, Puah C M, Joplin G F
Clin Chim Acta. 1977 Oct 15;80(2):285-91. doi: 10.1016/0009-8981(77)90035-3.
Levels of unconjugated testosterone, 5alpha-dihydrotestosterone, oestradiol and progesterone in human urine were measured before and after storage at various temperatures. Specific radioimmunoassays were used for all measurements. Levels did not change at -20 degrees C, and no change was detected at 4 degrees C for up to 5 days. Testosterone showed an average increase of 100% in 2-3 days at 18 degrees C and 5alpha-dihydrotestosterone about 200%. At 37 degrees C, the rise was two to three times faster still. The addition of enzyme inhibitors, bacteriostatic agents and various salts had no effect on the increase of the unconjugated androgens. Adjustment of the pH of samples to 2 caused about 4-fold rise of the androgens within 10 min, but at pH 8.6 there was no significant effect. Unconjugated oestradiol and progesterone levels showed a rise in a few samples at 18 degrees C for three days. At 37 degrees C both hormones showed a rise after 18 h in about two-thirds of samples, about 2-fold for progesterone and 6-fold for oestradiol.
在不同温度下储存前后，对人尿液中未结合睾酮、5α-双氢睾酮、雌二醇和孕酮的水平进行了测量。所有测量均采用特异性放射免疫分析法。在-20℃时水平未发生变化，在4℃下长达5天也未检测到变化。在18℃时，睾酮在2至3天内平均增加了100%，5α-双氢睾酮增加了约200%。在37℃时，上升速度还要快两到三倍。添加酶抑制剂、抑菌剂和各种盐对未结合雄激素的增加没有影响。将样品的pH值调至2会使雄激素在10分钟内升高约4倍，但在pH值为8.6时没有显著影响。未结合雌二醇和孕酮水平在18℃下放置三天后，在少数样品中出现升高。在37℃时，约三分之二的样品在18小时后两种激素均出现升高，孕酮升高约2倍，雌二醇升高约6倍。
