deCatanzaro D, Muir C, Beaton E, Jetha M, Nadella K
Department of Psychology, McMaster University, Hamilton, Ontario, Canada.
Reproduction. 2003 Sep;126(3):407-14. doi: 10.1530/rep.0.1260407.
ELISA measurements of 17beta-oestradiol, testosterone and progesterone were determined for urine samples collected non-invasively from female mice. Initial samples were collected during 5 successive days while mature female mice were isolated and cyclic. Subsequently, female mice were inseminated and additional urine samples were collected during days 2-6 after observation of copulatory plugs. Measurements of oestradiol and testosterone showed variance over days within individuals and did not significantly differ in measurements taken before or after insemination. Progesterone concentrations were significantly higher after insemination compared with before mating. In a second sample of inseminated females, urinary progesterone was measured during days 2-18 of pregnancy. Most females showed high urinary progesterone up to day 10 of pregnancy and lower concentrations during the remainder of gestation. These results indicate that urinary progesterone reflects established systemic increases of this hormone during pregnancy.
对从雌性小鼠非侵入性收集的尿液样本进行了17β - 雌二醇、睾酮和孕酮的酶联免疫吸附测定（ELISA）。最初的样本是在连续5天内收集的，此时成熟雌性小鼠处于隔离且有发情周期的状态。随后，对雌性小鼠进行授精，并在观察到交配栓后的第2 - 6天收集额外的尿液样本。雌二醇和睾酮的测量显示个体内不同天数存在差异，且授精前后的测量结果无显著差异。与交配前相比，授精后孕酮浓度显著升高。在授精雌性的第二个样本中，在妊娠的第2 - 18天测量尿孕酮。大多数雌性在妊娠第10天前尿孕酮含量较高，在妊娠剩余时间浓度较低。这些结果表明，尿孕酮反映了妊娠期间该激素在体内的既定升高。
