Robin G, Cohen D, Orr N, Markus I, Slepon R, Ashkenazi S, Keisari Y
Israel Defence Force, Medical Corps.
J Infect Dis. 1997 May;175(5):1128-33. doi: 10.1086/516452.
The IgG subclass response to Shigella sonnei and Shigella flexneri 2a lipopolysaccharide (LPS) was examined in subjects naturally exposed to these organisms. Affinity-purified LPS antibodies obtained using a column of Shigella LPS bound to epoxy-activated Sepharose 6B were used as standards to calibrate the serum antibody response to natural Shigella infection. The geometric mean concentrations of specific IgG in sera from those not exposed to Shigella organisms were 7.9 microg/mL against S. sonnei LPS and 18.6 microg/mL against S. flexneri 2a LPS. After natural exposure to S. sonnei or S. flexneri 2a, the concentrations rose to 30.3 and 127.9 microg/mL, respectively. IgG2 was the major component in the anti-S. flexneri subclass response, while the anti-S. sonnei response was dominated by IgG1. High levels of IgG1 antibodies before exposure to organisms from either Shigella serogroup correlated with a lower risk of developing symptomatic infection.
在自然接触这些细菌的受试者中，检测了对宋内志贺菌和福氏志贺菌2a型脂多糖（LPS）的IgG亚类反应。使用结合到环氧活化的琼脂糖凝胶6B上的志贺菌LPS柱获得的亲和纯化LPS抗体作为标准，以校准血清对自然志贺菌感染的抗体反应。未接触志贺菌的受试者血清中特异性IgG的几何平均浓度，针对宋内志贺菌LPS为7.9μg/mL，针对福氏志贺菌2a型LPS为18.6μg/mL。自然接触宋内志贺菌或福氏志贺菌2a后，浓度分别升至30.3和127.9μg/mL。IgG2是抗福氏志贺菌亚类反应的主要成分，而抗宋内志贺菌反应以IgG1为主。在接触任何一个志贺菌血清群的细菌之前，高水平的IgG1抗体与出现症状性感染的风险较低相关。
