Han Y, Cutler J E
Department of Microbiology, Montana State University, Bozeman 59717, USA.
J Infect Dis. 1997 May;175(5):1169-75. doi: 10.1086/516455.
As previously reported, monoclonal antibody (MAb) B6.1 increases resistance to hematogenous disseminated candidiasis in normal and SCID mice. In this study, MAb B6.1 was examined in a mouse model of neutropenia. The neutropenia was induced for a short period of time by a single dose of the anti-neutrophil antibody, MAb RB6-8C5, or for a protracted period by doses of MAb RB6-8C5 every other day. At low doses (< or = 25 microg/mouse), neutrophils were primarily affected, but at high doses (> or = 50 microg/mouse), lymphocytes were also depleted. Mice given either single or multiple doses of MAb RB6-8C5 were more resistant to experimental hematogenous disseminated candidiasis if they received MAb B6.1 before and after challenge with Candida albicans yeast cells intravenously. These results show the utility of MAb RB6-8C5 for induction of a protracted neutropenia in mice and demonstrate that MAb B6.1 can enhance resistance against candidiasis under neutropenic conditions.
如先前报道，单克隆抗体（MAb）B6.1可增强正常小鼠和重症联合免疫缺陷（SCID）小鼠对血源性播散性念珠菌病的抵抗力。在本研究中，在中性粒细胞减少的小鼠模型中对MAb B6.1进行了检测。通过单次注射抗中性粒细胞抗体MAb RB6-8C5在短时间内诱导中性粒细胞减少，或每隔一天注射MAb RB6-8C5在较长时间内诱导中性粒细胞减少。低剂量（≤25微克/小鼠）时，主要影响中性粒细胞，但高剂量（≥50微克/小鼠）时，淋巴细胞也会减少。如果在静脉注射白色念珠菌酵母细胞攻击前后给予MAb B6.1，接受单次或多次MAb RB6-8C5注射的小鼠对实验性血源性播散性念珠菌病更具抵抗力。这些结果表明MAb RB6-8C5在诱导小鼠长期中性粒细胞减少方面的效用，并证明MAb B6.1可在中性粒细胞减少的条件下增强对念珠菌病的抵抗力。
