Han Y, Cutler J E
Department of Microbiology, Montana State University, Bozeman 59717, USA.
Infect Immun. 1995 Jul;63(7):2714-9. doi: 10.1128/iai.63.7.2714-2719.1995.
We previously showed that surface mannans of Candida albicans function as adhesins during yeast cell attachment to mouse splenic marginal zone macrophages. The mannan adhesin fraction was encapsulated into liposomes and used to vaccinate mice over a 5- to 6-week period. Circulating agglutinins specific for the fraction correlated with increased resistance to disseminated candidiasis. Antiserum from vaccinated animals protected naive BALB/cByJ mice against C. albicans serotype A and B strains and Candida tropicalis. Antiserum also protected SCID mice against disseminated disease. The serum protective ability was stable at 56 degrees C, but this ability was adsorbed by C. albicans cells. The antiserum was divided into three fractions after separation by high-performance liquid chromatography. One fraction contained all of the agglutinin activity and transferred resistance to naive mice. A second fraction also transferred resistance. Two monoclonal antibodies (MAbs) specific for candidal surface determinants were obtained. MAb B6.1 is specific for a mannan epitope in the adhesin fraction, and MAb B6 is specific for a different epitope in the fraction. Both MAbs are immunoglobulin M, and both strongly agglutinate candidal cells, but only MAb B6.1 protected both normal and SCID mice against disseminated candidiasis. In one experiment, 10 normal mice were given MAb B6.1 and challenged with yeast cells. Six mice survived the 67-day observation period; 4 of the survivors were cured as evidenced by the lack of CFU in the kidney and spleen. Our studies show that antibodies against certain cell surface antigens of C. albicans help the host resist disseminated candidiasis.
我们先前表明，白色念珠菌的表面甘露聚糖在酵母细胞附着于小鼠脾脏边缘区巨噬细胞的过程中发挥黏附素的作用。将甘露聚糖黏附素部分包裹于脂质体中，并用于在5至6周的时间内给小鼠接种疫苗。针对该部分的循环凝集素与对播散性念珠菌病抵抗力的增强相关。来自接种动物的抗血清可保护未感染的BALB/cByJ小鼠抵御A型和B型血清型的白色念珠菌菌株以及热带念珠菌。抗血清还可保护SCID小鼠抵御播散性疾病。血清保护能力在56摄氏度时稳定，但这种能力可被白色念珠菌细胞吸附。通过高效液相色谱分离后，抗血清被分为三个部分。其中一个部分包含所有的凝集素活性，并将抵抗力传递给未感染的小鼠。第二个部分也传递了抵抗力。获得了两种针对念珠菌表面决定簇的单克隆抗体（MAb）。单克隆抗体B6.1对黏附素部分中的甘露聚糖表位具有特异性，单克隆抗体B6对该部分中的另一个不同表位具有特异性。两种单克隆抗体均为免疫球蛋白M，且都能强烈凝集念珠菌细胞，但只有单克隆抗体B6.1能保护正常小鼠和SCID小鼠抵御播散性念珠菌病。在一项实验中，给10只正常小鼠注射单克隆抗体B6.1，然后用酵母细胞进行攻击。6只小鼠在67天的观察期内存活；4只存活小鼠被治愈，肾脏和脾脏中缺乏菌落形成单位即可证明。我们的研究表明，针对白色念珠菌某些细胞表面抗原的抗体可帮助宿主抵抗播散性念珠菌病。
