Allen R C, Stevens P R, Price T H, Chatta G S, Dale D C
Department of Medicine, University of Washington, and Puget Sound Blood Center, Seattle, USA.
J Infect Dis. 1997 May;175(5):1184-92. doi: 10.1086/595866.
The effect of daily in vivo granulocyte colony-stimulating factor (G-CSF) treatment on neutrophil function was studied over a 14-day period using a luminescence system for differential measurement of oxidase and myeloperoxidase (MPO) dioxygenation activities in whole blood. Opsonin receptor-mediated phagocyte functions were also measured with this system. G-CSF produced a dose-dependent neutrophil leukocytosis and a proportional increase in oxidase activity per volume of blood. The oxidase activity per neutrophil remained relatively constant throughout the test period. However, both chemical- and opsonin-stimulated MPO oxygenation activities per neutrophil were greatly increased by treatment with maxima correlating temporally to initial G-CSF exposure during the early mitotic phase of neutrophil development. The possibility that peroxynitrite contributes to this maximum luminol-dependent activity was tested, but neither superoxide dismutase, a competitive inhibitor of peroxynitrite production, nor N-methyl-L-arginine, an inhibitor of nitric oxide synthase, exerted a significant inhibitory effect.
使用发光系统在14天的时间里研究了每日体内注射粒细胞集落刺激因子（G-CSF）对中性粒细胞功能的影响，该系统用于差异测量全血中的氧化酶和髓过氧化物酶（MPO）双加氧活性。还用该系统测量了调理素受体介导的吞噬细胞功能。G-CSF产生了剂量依赖性的中性粒细胞增多症，并且每单位体积血液中的氧化酶活性成比例增加。在整个测试期间，每个中性粒细胞的氧化酶活性保持相对恒定。然而，通过治疗，每个中性粒细胞的化学刺激和调理素刺激的MPO氧化活性都大大增加，其最大值在中性粒细胞发育的早期有丝分裂阶段与最初的G-CSF暴露在时间上相关。测试了过氧亚硝酸盐促成这种最大的鲁米诺依赖性活性的可能性，但过氧亚硝酸盐产生的竞争性抑制剂超氧化物歧化酶和一氧化氮合酶抑制剂N-甲基-L-精氨酸均未发挥显著的抑制作用。
