Ito M, Watanabe M, Kamiya H, Sakurai M
Department of Pediatrics, Mie University School of Medicine, Japan.
J Infect Dis. 1997 May;175(5):1220-4. doi: 10.1086/593672.
Apoptosis in peripheral blood T lymphocytes induced by herpes simplex virus type 1 (HSV-1) was investigated by use of DNA content analysis and the terminal deoxytransferase-mediated dUTP nick end-labeling method. The hypodiploid fraction in the cell cycle and DNA fragmentation were both increased in HSV-1-infected T lymphocytes compared with that in mock-infected T lymphocytes on stimulation with phytohemagglutinin (PHA). There was no difference in the hypodiploid fraction and DNA fragmentation between HSV-1-infected T lymphocytes and mock-infected cells cultured without PHA. HSV-1 enhanced apoptosis in CD4 and HLA-DR-positive T lymphocytes, but not in CD8 lymphocytes, when stimulated with PHA. These results demonstrate that HSV-1 infection enhances apoptosis in activated T lymphocytes, particularly CD4 and HLA-DR-positive T lymphocytes. bcl-2 expression was unchanged in mock- and HSV-1-infected CD4 and CD8 lymphocytes cultured with or without PHA, indicating that bcl-2 does not contribute to HSV-1-induced apoptotic cell death. Apoptosis in CD4 helper T lymphocytes may account for immunosuppression and lymphocytopenia following HSV-1 infection.
运用DNA含量分析及末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法，对单纯疱疹病毒1型（HSV-1）诱导的外周血T淋巴细胞凋亡进行了研究。与用植物血凝素（PHA）刺激的模拟感染T淋巴细胞相比，HSV-1感染的T淋巴细胞在细胞周期中的亚二倍体比例及DNA片段化均增加。在未用PHA培养的情况下，HSV-1感染的T淋巴细胞与模拟感染细胞之间的亚二倍体比例及DNA片段化无差异。当用PHA刺激时，HSV-1增强了CD4及HLA-DR阳性T淋巴细胞的凋亡，但未增强CD8淋巴细胞的凋亡。这些结果表明，HSV-1感染增强了活化T淋巴细胞尤其是CD4及HLA-DR阳性T淋巴细胞的凋亡。在用或不用PHA培养的模拟感染及HSV-1感染的CD4及CD8淋巴细胞中，bcl-2表达均未改变，表明bcl-2不参与HSV-1诱导的凋亡性细胞死亡。CD4辅助性T淋巴细胞的凋亡可能是HSV-1感染后免疫抑制及淋巴细胞减少的原因。
