Wang H L, Jin D Y
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing.
J Infect Dis. 1997 May;175(5):1229-33. doi: 10.1086/593676.
Prevalence of hepatitis G virus (HGV) was determined in a cohort of Chinese blood donors and hepatitis patients by the detection of viral RNA via reverse transcription-polymerase chain reaction. While HGV RNA was detected in only 1 of 150 healthy volunteers, the detection rate among professional blood donors was surprisingly high (21/265, 7.9%), and plasmapheresis was identified as a significant risk factor in this population. It was also shown that an elevated serum alanine aminotransferase level is not a reliable marker for HGV infection. Prevalences of HGV in patients with hepatitis C, with non-A-E hepatitis, and with hepatocellular carcinoma were relatively low (8.2%, 16.7%, and 6.1%, respectively). Striking sequence homology (>90%) shared by 5 HGV cDNA clones implicated that they belonged to the same genotype. Phylogenetic analysis of a 446-bp NS3 cDNA confirmed that this genotype was closely related to the prototype viruses.
通过逆转录聚合酶链反应检测病毒RNA，在中国献血者和肝炎患者队列中确定庚型肝炎病毒（HGV）的流行情况。虽然在150名健康志愿者中仅1人检测到HGV RNA，但职业献血者中的检出率惊人地高（265人中有21人，7.9%），血浆置换被确定为该人群中的一个重要危险因素。研究还表明，血清丙氨酸氨基转移酶水平升高并非HGV感染的可靠标志物。丙型肝炎患者、非甲-戊型肝炎患者和肝细胞癌患者中HGV的流行率相对较低（分别为8.2%、16.7%和6.1%）。5个HGV cDNA克隆具有显著的序列同源性（>90%），这表明它们属于同一基因型。对一段446bp的NS3 cDNA进行系统发育分析证实，该基因型与原型病毒密切相关。
