Vårum K M, Myhr M M, Hjerde R J, Smidsrød O
Norwegian Biopolymer Laboratory (NOBIPOL), Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway.
Carbohydr Res. 1997 Mar 26;299(1-2):99-101. doi: 10.1016/s0008-6215(96)00332-1.
The initial degradation rates (r) in human serum of three chitosans with FA = 0.42, 0.51, and 0.60 were determined by measuring the decrease in viscosity as a function of time. A strong increase in r with increasing FA of the chitosans was observed, with r increasing proportionally to FA4.5. With increasing concentrations of lysozyme added to the reaction mixtures of chitosan and serum, the relative increase in degradation rate of chitosans with increasing FA was almost the same as that without lysozyme added. Addition of the chitinase inhibitor allosamidin (50 microM) did not inhibit the degradation rate of chitosan (FA = 0.60) by human serum. The results suggest that chitosans are actually mainly depolymerized by lysozyme in human serum, and not by other enzymes or other depolymerization mechanisms.
通过测量粘度随时间的降低情况，测定了三种脱乙酰度（FA）分别为0.42、0.51和0.60的壳聚糖在人血清中的初始降解速率（r）。观察到随着壳聚糖脱乙酰度的增加，降解速率显著提高，降解速率与脱乙酰度的4.5次方成正比。随着添加到壳聚糖与血清反应混合物中的溶菌酶浓度增加，脱乙酰度增加时壳聚糖降解速率的相对增加与未添加溶菌酶时几乎相同。添加几丁质酶抑制剂别藻霉素（50微摩尔）并未抑制人血清对壳聚糖（脱乙酰度 = 0.60）的降解速率。结果表明，壳聚糖在人血清中实际上主要是被溶菌酶解聚，而非其他酶或其他解聚机制。
