[采用阿尔辛蓝对包埋于环氧树脂中的牙组织超薄切片进行酸性黏多糖检测]
[Acid mucopolysaccharide detection on ultra-thin sections by alcian blue in dental tissues embedded in Epon].
作者信息
Goldberg M, Triller M, Escaig F, Genotelle-Septier D, Weill R
出版信息
J Biol Buccale. 1976 Jun;4(2):155-64.
Ultra-thin sections of dental tissues, fixed in glutaraldehyde and osmium tetroxide, embedded without previous demineralization in Epon, were contrasted the grids with an alcian blue solution after previous oxidation periodic acid or hydrogen peroxide. Correlations with histochemical results obtained with the optical microscope, other results obtained with ruthenium red and phosphotungstic acid and controls made after enzymatic digestion with chondroitinase AC suggest that this technique is specific for the detection of acid mucopolysaccharides in those calcified tissues studied.
将固定在戊二醛和四氧化锇中的牙齿组织超薄切片，未经预先脱矿质处理直接包埋在环氧树脂中，在经过高碘酸或过氧化氢氧化处理后，用阿尔新蓝溶液对网格进行对比染色。与光学显微镜下获得的组织化学结果、钌红和磷钨酸获得的其他结果以及用软骨素酶AC进行酶消化后所做的对照结果之间的相关性表明，该技术对于在所研究的那些钙化组织中检测酸性粘多糖具有特异性。
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