Cardoni R L, Antunez M I, Morales C, Nantes I R
Instituto Nacional De Chagas, Dr. M. Fatala Chaben, Buenos Aires, Argentina.
Am J Trop Med Hyg. 1997 Mar;56(3):329-34. doi: 10.4269/ajtmh.1997.56.329.
The release of reactive oxygen intermediates (ROI), mediators of inflammatory reactions, was evaluated in murine Trypanosoma cruzi infection. In acutely infected BALB/c mice, spleen cells were stimulated, either with epimastigote or trypomastigote forms of the parasite, and the effect was enhanced by serum from infected mice. Only opsonized parasites triggered the release of ROI by normal mouse cells and this response was several times lower than in infected mice. This seems to indicate that cells from acutely infected mice reacted to T. cruzi and that neither parasites nor serum factors blocked the release of ROI. During the acute stage of the infection, both the parasitemia and the release of ROI by spleen cells were higher in BALB/c than in C3H mice (ROI generated in response to a phagocytic stimulation was 12 and 3 times the normal levels, respectively). In addition, in BALB/c mice infected with different numbers of parasites, the production of ROI was related to parasitemia. On the other hand, during the chronic stage of the infection, the inflammatory reaction in myocardium was greater in C3H than in BALB/c mice, and the increase in ROI production was 30% and 100% above the normal levels in BALB/c and C3H mice, respectively. This suggests that the increased ROI production paralleled the parasite burden in the acute phase, and could be related to inflammatory processes after the control of the parasitemia.
在小鼠克氏锥虫感染中评估了活性氧中间体（ROI）的释放情况，ROI是炎症反应的介质。在急性感染的BALB/c小鼠中，用寄生虫的上鞭毛体或锥鞭毛体形式刺激脾细胞，感染小鼠的血清可增强这种效应。只有经调理素作用的寄生虫能触发正常小鼠细胞释放ROI，且这种反应比感染小鼠低几倍。这似乎表明急性感染小鼠的细胞对克氏锥虫有反应，寄生虫和血清因子均未阻断ROI的释放。在感染的急性期，BALB/c小鼠的寄生虫血症和脾细胞释放ROI的水平均高于C3H小鼠（对吞噬刺激产生的ROI分别是正常水平的12倍和3倍）。此外，在感染不同数量寄生虫的BALB/c小鼠中，ROI的产生与寄生虫血症相关。另一方面，在感染的慢性期，C3H小鼠心肌的炎症反应比BALB/c小鼠更强烈，BALB/c和C3H小鼠中ROI产生的增加分别比正常水平高30%和100%。这表明在急性期ROI产生的增加与寄生虫负荷平行，并且可能与寄生虫血症得到控制后的炎症过程有关。
