Martínez-Augustin O, Boza J J, Del Pino J I, Lucena J, Martínez-Valverde A, Gil A
Department of Biochemistry and Molecular Biology, University of Granada, Spain.
Biol Neonate. 1997;71(4):215-23. doi: 10.1159/000244420.
The objective of this study was to evaluate the influence of dietary nucleotide supplementation in preterm infants during the first month of life on the intestinal permeability to lactulose, mannitol and to beta-lactoglobulin and on the development of circulating antibodies to beta-lactoglobulin and alpha-casein. Twenty-seven preterm infants were enrolled in the study; 11 of them were fed a standard low-birth weight milk formula and 16 infants were fed the same formula supplemented with nucleotides at similar levels to those found in human milk. Blood and urine samples were obtained at 1, 7 and 30 days of age. Serum beta-lactoglobulin, serum IgG antibody to alpha-casein and serum IgG antibody to beta-lactoglobulin were measured by ELISA. The lactulose/mannitol urinary excretion rate was measured by gas liquid chromatography. Neither the intestinal permeability to saccharides nor the intestinal absorption of beta-lactoglobulin were affected by the nucleotide supplementation. However, serum concentrations of IgG antibody to beta-lactoglobulin were higher in preterm neonates fed the supplemented formula than in those fed the standard formula. According to these results, dietary nucleotides might influence the maturation of the humoral immune response in preterm newborn infants.
本研究的目的是评估出生后第一个月内给早产儿补充膳食核苷酸对其肠道对乳果糖、甘露醇和β-乳球蛋白的通透性以及对β-乳球蛋白和α-酪蛋白循环抗体产生的影响。27名早产儿参与了本研究；其中11名喂食标准低体重儿奶粉，16名喂食添加了与母乳中含量相似水平核苷酸的相同奶粉。在出生1天、7天和30天时采集血样和尿样。采用酶联免疫吸附测定法（ELISA）检测血清β-乳球蛋白、抗α-酪蛋白血清IgG抗体和抗β-乳球蛋白血清IgG抗体。采用气液色谱法测定乳果糖/甘露醇尿排泄率。补充核苷酸对肠道对糖类的通透性和β-乳球蛋白的肠道吸收均无影响。然而，喂食添加配方奶粉的早产新生儿血清中抗β-乳球蛋白IgG抗体浓度高于喂食标准配方奶粉的新生儿。根据这些结果，膳食核苷酸可能会影响早产新生儿体液免疫反应的成熟。
