Triggiani M, De Marino V, de Crescenzo G, Marone G
Division of Clinical Immunology and Allergy, University of Naples Federico II, Italy.
Int Arch Allergy Immunol. 1997 May-Jul;113(1-3):190-2. doi: 10.1159/000237543.
Recent evidence suggests that arachidonic acid (AA), the precursor of eicosanoids, is stored in various glycerolipid pools with different biochemical specificities. Upon cell activation, AA is rapidly remodeled within these glycerolipid pools. We have explored the changes in AA content and distribution in human neutrophils as they are activated in vivo in the lungs of patients with adult respiratory distress syndrome (ARDS). Neutrophils from bronchoalveolar lavage fluid of ARDS patients contained an amount of total cellular AA four times larger than that of blood (resting) neutrophils and accumulated a larger proportion of AA into a pool associated with triglycerides (TG). These biochemical changes were associated with an increased number of cytoplasmic lipid bodies and with the acquisition of the hypodense phenotype. These data indicate that the accumulation of AA into TG is a marker of cell activation and suggest a central role of the TG pool in AA metabolism in inflammatory cells activated in vivo.
最近的证据表明，花生四烯酸（AA）作为类二十烷酸的前体，储存于具有不同生化特性的各种甘油脂质池中。细胞激活后，AA在这些甘油脂质池中迅速重塑。我们探讨了成年呼吸窘迫综合征（ARDS）患者肺部体内激活时，人中性粒细胞中AA含量和分布的变化。ARDS患者支气管肺泡灌洗 fluid中的中性粒细胞所含的总细胞AA量比血液（静息）中性粒细胞大四倍，并且将更大比例的AA积累到与甘油三酯（TG）相关的池中。这些生化变化与细胞质脂质体数量增加以及低密度表型的获得有关。这些数据表明，AA积累到TG中是细胞激活的标志，并提示TG池在体内激活的炎症细胞中AA代谢中起核心作用。
