Sillem M, Prifti S, Schmidt M, Rabe T, Runnebaum B
Department of Obstetrics and Gynecology, Ruprecht-Karls-Universitaet, Heidelberg, Germany.
Fertil Steril. 1997 May;67(5):877-82. doi: 10.1016/s0015-0282(97)81400-8.
To examine the regulation of endometrial integrin expression by estrogens and progestins in vitro.
Immunocytochemical study.
Academic research unit.
PATIENT(S): Twenty-five regularly cycling women without endometrial pathology, of whom seven had endometriosis.
INTERVENTION(S): Endometrial cells obtained by aspiration curettage were treated with diethylstilbestrol, promegestone, and antiprogestin. Immunocytochemistry was performed with antibodies directed against integrins alpha 1 beta 1, alpha 2 beta 1, alpha 4 beta 1, alpha 5 beta 1, alpha v beta 3, and beta 3 integrin subunit.
MAIN OUTCOME MEASURE(S): Semiquantitative staining score.
RESULT(S): Endometrial cells express several integrins in vitro in a consistent and cell specific pattern. Neither differences between treated and untreated cells nor an effect of treatment duration or dosage were observed. Cells from patients with and without endometriosis showed similar patterns.
CONCLUSION(S): The cellular distribution of integrin expression was similar to that described in vivo. In contrast, a steroid regulated expression could not be detected in vitro. Rather, a derepression by a factor not included in our model could be responsible for the cyclic appearance of some integrins. In endometriosis, no fundamental difference of integrin expression was detected.
在体外研究雌激素和孕激素对子宫内膜整合素表达的调节作用。
免疫细胞化学研究。
学术研究单位。
25名月经周期正常且无子宫内膜病变的女性，其中7名患有子宫内膜异位症。
通过刮宫吸取获得的子宫内膜细胞用己烯雌酚、普美孕酮和抗孕激素进行处理。使用针对整合素α1β1、α2β1、α4β1、α5β1、αvβ3和β3整合素亚基的抗体进行免疫细胞化学检测。
半定量染色评分。
子宫内膜细胞在体外以一致且细胞特异性的模式表达多种整合素。未观察到处理组和未处理组细胞之间的差异，也未观察到处理持续时间或剂量的影响。患有和未患有子宫内膜异位症的患者的细胞表现出相似的模式。
整合素表达的细胞分布与体内描述的相似。相比之下，在体外未检测到类固醇调节的表达。相反，我们模型中未包含的一种因子的去抑制作用可能是某些整合素周期性出现的原因。在子宫内膜异位症中，未检测到整合素表达的根本差异。
