Béliard A, Donnez J, Nisolle M, Foidart J M
Laboratory of Biology, University of Liège, Belgium.
Fertil Steril. 1997 Feb;67(2):266-72. doi: 10.1016/S0015-0282(97)81909-7.
To compare the localization of adhesion proteins (laminin and fibronectin) and their receptors of the integrin family in endometriosis and endometrium.
An immunohistochemical study.
University Hospital, Department of Gynecology and Department of Cell Biology.
PATIENT(S): Eighteen endometriosis patients undergoing laparoscopy for pain or infertility and nine control patients undergoing laparoscopy for sterilization or hysterectomy.
MAIN OUTCOME MEASURE(S): The expression of adhesion glycoproteins (laminin and fibronectin), their receptors alpha 1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 5 beta 1, and alpha 6 beta 1, and E-cadherin was determined by immunohistochemistry on frozen sections.
RESULT(S): The distribution of both adhesive glycoproteins, laminin and fibronectin, and their receptors was identical in endometriosis and endometrium. Fibronectin receptors (alpha 4 beta 1, alpha 5 beta 1) displayed distinct expression patterns in endometrium and endometriosis. No endometrial glands showed positive staining for the alpha 5 chain, whereas this integrin subunit was detected in almost all endometriotic lesions. The integrin alpha 4 chain was present in all endometriotic glands but was absent from endometrial glands in the proliferative phase of the cycle.
CONCLUSION(S): No difference in cell adhesion molecule localization nor receptors was observed between endometriotic and endometrial samples, except for fibronectin receptors. Their expression persisted around endometriotic glands but not in endometrium. These results suggest that fibronectin receptors could play a role in the persistence of endometriotic lesions, despite menstruation in corresponding eutopic endometrium.
比较子宫内膜异位症和子宫内膜中黏附蛋白（层粘连蛋白和纤连蛋白）及其整合素家族受体的定位。
一项免疫组织化学研究。
大学医院，妇科和细胞生物学系。
18例因疼痛或不孕接受腹腔镜检查的子宫内膜异位症患者，以及9例因绝育或子宫切除术接受腹腔镜检查的对照患者。
通过对冰冻切片进行免疫组织化学测定黏附糖蛋白（层粘连蛋白和纤连蛋白）、其受体α1β1、α2β1、α3β1、α5β1和α6β1以及E-钙黏蛋白的表达。
层粘连蛋白和纤连蛋白这两种黏附糖蛋白及其受体在子宫内膜异位症和子宫内膜中的分布相同。纤连蛋白受体（α4β1、α5β1）在子宫内膜和子宫内膜异位症中表现出不同的表达模式。没有子宫内膜腺体显示α5链呈阳性染色，而在几乎所有子宫内膜异位病变中都检测到了这种整合素亚基。整合素α4链存在于所有子宫内膜异位腺体中，但在月经周期增殖期的子宫内膜腺体中不存在。
除纤连蛋白受体外，在子宫内膜异位症和子宫内膜样本之间未观察到细胞黏附分子定位及其受体的差异。它们的表达在子宫内膜异位腺体周围持续存在，但在子宫内膜中不存在。这些结果表明，尽管相应正常子宫内膜会发生月经，但纤连蛋白受体可能在子宫内膜异位病变的持续存在中起作用。
