Vlachos V, Critchley A T, von Holy A
Department of Microbiology, University of the Witwatersrand, Johannesburg, South Africa.
Microbios. 1996;88(355):115-23.
Six southern African seaweeds, two Chlorophyta, Phaeophyta and Rhodophyta, were used to test for antibacterial activity against Bacillus subtilis, Staphylococcus aureus, Acinetobacter Iwoffi and Escherichia coli. Extracts were prepared using a range of organic solvents (acetone, chloroform, diethyl ether, 80% ethanol and methanol), by soaking ground seaweed samples in the extraction solvent, evaporating to dryness, and redissolving in the respective solvent. In addition, milled and ground samples were boiled in 80% ethanol for 4 h. Crude extracts were tested for antibacterial activity using three application techniques, viz paper discs, direct spotting and wells, and onto three types of agar plates, viz seeded agar, bacterial lawns and overlay agar. Inhibition zone diameters were measured and analysed by multifactorial analysis of variance. Boiling milled seaweeds in 80% ethanol, with application of the extracts into 6.1 mm wells in overlay agar constituted the test protocol which yielded the highest activity indices.
六种来自南部非洲的海藻，包括两种绿藻、褐藻和红藻，被用于测试其对枯草芽孢杆菌、金黄色葡萄球菌、沃氏不动杆菌和大肠杆菌的抗菌活性。通过将研磨后的海藻样品浸泡在一系列有机溶剂（丙酮、氯仿、乙醚、80%乙醇和甲醇）中，蒸发至干，然后再溶解于相应溶剂中来制备提取物。此外，将研磨后的样品在80%乙醇中煮沸4小时。使用三种应用技术，即纸片法、直接点滴法和打孔法，在三种类型的琼脂平板上，即接种琼脂平板、细菌菌苔平板和覆盖琼脂平板上，对粗提物进行抗菌活性测试。测量抑菌圈直径，并通过多因素方差分析进行分析。在80%乙醇中煮沸研磨后的海藻，并将提取物应用于覆盖琼脂平板上6.1毫米的孔中，构成了产生最高活性指数的测试方案。
