Bray B A
Ann N Y Acad Sci. 1978 Jun 20;312:142-50. doi: 10.1111/j.1749-6632.1978.tb16799.x.
Cold-insoluble globulin was detected in both trophoblast and alveolar basement membrane preparations, whereas it was not detected in GBM preparations. Acidic structural glycoproteins from both placental villi and lung parenchyma, which were extracted with 0.3 M acetic acid and recovered by adjusting the pH to 4.7, also contained CIg. Fractions of TBM, solubilized by either dilute alkali (0.01 N NaOH), by reduction and alkylation of the disulfide bonds, or by 0.3 M acetic acid extraction, all contained the antigen and possessed properties similar to those of ASG. The ASG fractions also reacted with antifibrinogen, but proof that the two types of determinants occur on a given molecular species is lacking at present. Purified collagenase solubilized CIg from ABM, from lung parenchyma, and from the stroma of placental villi, and this finding is strong evidence for an association of CIg with collagen in these connective tissues.
在滋养层和肺泡基底膜制剂中均检测到冷不溶性球蛋白，而在肾小球基底膜制剂中未检测到。用0.3M乙酸提取并通过将pH值调至4.7回收的来自胎盘绒毛和肺实质的酸性结构糖蛋白也含有冷不溶性球蛋白。经稀碱（0.01N NaOH）、二硫键还原烷基化或0.3M乙酸提取溶解的基底膜成分均含有该抗原，且具有与酸性结构糖蛋白相似的性质。酸性结构糖蛋白成分也与抗纤维蛋白原发生反应，但目前缺乏证据证明这两种类型的决定簇存在于同一分子上。纯化的胶原酶可溶解来自肺泡基底膜、肺实质和胎盘绒毛基质中的冷不溶性球蛋白，这一发现有力地证明了冷不溶性球蛋白与这些结缔组织中的胶原蛋白有关联。
