Mark M A, Jarrott B, Colvin L A, MacMillan S J, Duggan A W
Department of Preclinical Veterinary Sciences, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Summerhall, UK.
Brain Res. 1997 Apr 18;754(1-2):195-203. doi: 10.1016/s0006-8993(97)00061-9.
The release of immunoreactive (ir-) neuropeptide Y (NYP) was studied in the anaesthetized rat and cat by means of microprobes bearing immobilized antibodies to the C terminus of NPY. An extensive basal release of ir-NYP was detected throughout the dorsal and upper ventral horn of the rat. This spontaneous release was not significantly altered by sectioning the spinal cord at the thoraco-lumbar junction nor by electrical stimulation of peripheral nerves. Since NPY is virtually absent in primary afferents it is probable that spontaneous release within the spinal cord comes from active NPY-containing intrinsic spinal neurones. In the spinal cat spontaneous release of ir-NPY was detected in the mid-dorsal horn and this was unaltered by peripheral noxious thermal or noxious mechanical stimuli. As in the rat, release from intrinsic spinal neurones is most probable. The extensive spontaneous release of ir-NPY in both species suggests a widespread role in spinal cord function.
利用携带针对神经肽Y（NPY）C末端的固定抗体的微探针，在麻醉的大鼠和猫中研究了免疫反应性（ir-）神经肽Y（NYP）的释放。在大鼠整个背角和腹角上部检测到大量基础ir-NYP释放。切断胸腰段脊髓或电刺激外周神经后，这种自发释放没有显著改变。由于初级传入纤维中几乎不存在NPY，脊髓内的自发释放可能来自活跃的含NPY的脊髓固有神经元。在脊髓猫的背角中部检测到ir-NPY的自发释放，外周有害热刺激或有害机械刺激不会改变这种释放。与大鼠一样，最有可能是脊髓固有神经元释放的。两种动物中ir-NPY的广泛自发释放表明其在脊髓功能中具有广泛作用。
