Duggan A W, Hope P J, Lang C W, Williams C A
Department of Preclinical Veterinary Sciences, University of Edinburgh, U.K.
Neurosci Lett. 1991 Jan 28;122(2):191-4. doi: 10.1016/0304-3940(91)90855-n.
Antibody microprobes were used to study release of immunoreactive neurokinins in the dorsal horn of the anaesthetized spinal cat following sustained isometric contraction of ipsilateral hindlimb muscles. Microprobes had immobilized antibodies to neurokinin A (NKA) on their outer surfaces and bound a proportion of released molecules when inserted in the central nervous system. Bound molecules were detected in autoradiographs as zones of reduced binding of 125I-NKA in which microprobes were incubated after withdrawal from the spinal cord. The left hindlimb was immobilized using an epoxy bandage splint and isometric contraction of muscles induced by intermittent tetanic stimulation of a ventral root. A basal presence of immunoreactive neurokinins was detected and this was increased by sustained isometric muscle contraction. It is probable that ergoreceptors contain and release neurokinins.
抗体微探针用于研究麻醉的脊髓猫在同侧后肢肌肉持续等长收缩后，背角中免疫反应性神经激肽的释放情况。微探针的外表面固定有抗神经激肽A（NKA）的抗体，当插入中枢神经系统时，能结合一部分释放的分子。在从脊髓取出微探针后，将其与125I-NKA一起孵育，在放射自显影片中，结合分子被检测为125I-NKA结合减少的区域。用环氧绷带夹板固定左后肢，通过对腹根进行间歇性强直刺激诱导肌肉等长收缩。检测到免疫反应性神经激肽的基础存在，并且这种存在会因持续的等长肌肉收缩而增加。很可能本体感受器含有并释放神经激肽。
