Heyer B S, Warsowe J, Solter D, Knowles B B, Ackerman S L
Max-Planck-Institut, Freiburg, Germany.
Mol Reprod Dev. 1997 Jun;47(2):148-56. doi: 10.1002/(SICI)1098-2795(199706)47:2<148::AID-MRD4>3.0.CO;2-M.
The initial phase of mammalian preimplantation development is directed by stored maternal mRNAs and their encoded proteins, yet most of the molecules controlling this process have not been described. We have used differential display analysis of cDNA libraries prepared from unfertilized eggs and preimplantation embryos to isolate three maternal cDNAs that represent novel genes exhibiting different patterns of expression during this developmental period. One of these, Melk, encodes a protein with a kinase catalytic domain and a leucine zipper motif, a new member of the Snf1/AMPK family of kinases. This gene product may play a role in the signal transduction events in the egg and early embryo.
哺乳动物着床前发育的初始阶段由储存的母体mRNA及其编码的蛋白质主导，然而，控制这一过程的大多数分子尚未得到描述。我们利用从未受精卵和着床前胚胎制备的cDNA文库进行差异显示分析，分离出三个母体cDNA，它们代表了在这一发育阶段呈现不同表达模式的新基因。其中一个基因Melk编码一种具有激酶催化结构域和亮氨酸拉链基序的蛋白质，它是Snf1/AMPK激酶家族的新成员。该基因产物可能在卵子和早期胚胎的信号转导事件中发挥作用。
