Heyer B S, Kochanowski H, Solter D
Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
Dev Dyn. 1999 Aug;215(4):344-51. doi: 10.1002/(SICI)1097-0177(199908)215:4<344::AID-AJA6>3.0.CO;2-H.
A new gene named maternal embryonic leucine zipper kinase, Melk, has been recently identified (Heyer et al. [1997] Mol. Reprod. Dev. 47:148-156). As a basis for further study of the function of the gene, we have examined the expression of Melk across a wide range of embryonic stages, from the ovulated egg and 2-cell embryo through the gastrulation and early organogenesis stages, by in situ hybridization and immunohistochemistry. Melk is expressed in a spatially and temporally specific pattern during mammalian embryogenesis. The strongest expression was detected during maturation of oocytes and preimplantation development. Given its expression pattern, Melk may play an important role during preimplantation embryonic development. Dev Dyn 1999;215:344-351.
最近发现了一个名为母体胚胎亮氨酸拉链激酶（Melk）的新基因（Heyer等人，[1997]《分子生殖与发育》47:148 - 156）。作为进一步研究该基因功能的基础，我们通过原位杂交和免疫组织化学技术，检测了Melk在从排卵卵和2细胞胚胎到原肠胚形成及早期器官发生阶段等广泛胚胎时期的表达情况。Melk在哺乳动物胚胎发育过程中呈现出时空特异性表达模式。在卵母细胞成熟和植入前发育阶段检测到最强表达。鉴于其表达模式，Melk可能在植入前胚胎发育过程中发挥重要作用。《发育动力学》1999年；215:344 - 351。
