Mori K, Yoneya S, Hayashi N, Abe T, Isono H, Nishiyama Y
Department of Ophthalmology, Saitama Medical School, Japan.
Nippon Ganka Gakkai Zasshi. 1997 Apr;101(4):318-26.
We performed angiography in photocoagulated eyes using four fluorescent dyes that have different molecular weight, lipid solubility, and electric charge. These findings were compared with those of sodium fluorescein (Naf) and indocyanine green (ICG). Angiograms were obtained with Naf, ICG and two fluorescein isothiocyanate dextrans at different molecular weight (FITC-D4: MW 4,400, FITC-D40: MW 38,900). We also used two other dyes: carboxyfluorescein (Caf: less lipid soluble) and calcein (Calc: a highly negatively charged molecule). Two and four days after laser photocoagulation of retina, dye leakage was still evident at the photocoagulated sites with Naf, Caf, Calc and FITC-D4. Angiograms taken after Caf and Calc injection showed more intense and rapid dye leakage than with Naf. Contrary to this, little dye leakage was observed with ICG and FITC-D40. These findings indicated that the critical molecular weight for permeation lay between 4,400 and 38,900 once the breakdown of blood retinal barrier occurred. In addition, difference in molecular weight is a major factor determining dye permeability at the photocoagulated retinal lesions, among other possibilities such as lipid solubility and electric charge.
我们使用四种具有不同分子量、脂溶性和电荷的荧光染料，对接受光凝治疗的眼睛进行血管造影。将这些结果与荧光素钠（Naf）和吲哚菁绿（ICG）的结果进行比较。使用Naf、ICG以及两种不同分子量的异硫氰酸荧光素葡聚糖（FITC-D4：分子量4400，FITC-D40：分子量38900）获得血管造影片。我们还使用了另外两种染料：羧基荧光素（Caf：脂溶性较低）和钙黄绿素（Calc：一种带高度负电荷的分子）。视网膜激光光凝术后两天和四天，在接受光凝治疗的部位，Naf、Caf、Calc和FITC-D4仍有明显的染料渗漏。注射Caf和Calc后拍摄的血管造影片显示，染料渗漏比Naf更强烈、更迅速。与此相反，ICG和FITC-D40几乎没有观察到染料渗漏。这些结果表明，一旦血视网膜屏障被破坏，渗透的临界分子量介于4400和38900之间。此外，分子量差异是决定光凝视网膜病变处染料渗透性的主要因素，其他可能因素如脂溶性和电荷也有影响。
