Nicolelis M A, Ghazanfar A A, Faggin B M, Votaw S, Oliveira L M
Department of Neurobiology, Duke University Medical School, Durham, North Carolina 27710, USA.
Neuron. 1997 Apr;18(4):529-37. doi: 10.1016/s0896-6273(00)80295-0.
Little is known about the physiological principles that govern large-scale neuronal interactions in the mammalian brain. Here, we describe an electrophysiological paradigm capable of simultaneously recording the extracellular activity of large populations of single neurons, distributed across multiple cortical and subcortical structures in behaving and anesthetized animals. Up to 100 neurons were simultaneously recorded after 48 microwires were implanted in the brain stem, thalamus, and somatosensory cortex of rats. Overall, 86% of the implanted microwires yielded single neurons, and an average of 2.3 neurons were discriminated per microwire. Our population recordings remained stable for weeks, demonstrating that this method can be employed to investigate the dynamic and distributed neuronal ensemble interactions that underlie processes such as sensory perception, motor control, and sensorimotor learning in freely behaving animals.
关于支配哺乳动物大脑中大规模神经元相互作用的生理原理，我们所知甚少。在此，我们描述了一种电生理范式，它能够在行为动物和麻醉动物中，同时记录分布于多个皮质和皮质下结构的大量单个神经元的细胞外活动。在将48根微丝植入大鼠脑干、丘脑和体感皮层后，可同时记录多达100个神经元。总体而言，86%的植入微丝记录到了单个神经元，每根微丝平均分辨出2.3个神经元。我们的群体记录在数周内保持稳定，表明该方法可用于研究自由行为动物中诸如感觉感知、运动控制和感觉运动学习等过程背后的动态和分布式神经元群体相互作用。
