Avet-Loiseau H, Vigier M, Moreau A, Mellerin M P, Gaillard F, Harousseau J L, Bataille R, Milpied N
Laboratory of Haematology, University Hospital, Nantes, France.
Br J Haematol. 1997 Apr;97(1):119-22. doi: 10.1046/j.1365-2141.1997.d01-2140.x.
Comparative genomic hybridization (CGH) was used to analyse 34 follicular lymphoma (FL) samples. 27 samples showed DNA sequence copy number changes of at least one genomic region (26 samples with at least one gain and nine with at least one loss). Some chromosomes or chromosomal regions were preferentially involved. The most frequently gained regions were chromosome 18q (29% of samples), chromosome X (21%), chromosome 7 (18%), chromosomes 2, 6p and 8q (12%). Two regions were preferentially lost: 6q (12%) and 17p (9%). All these gained and lost regions have been previously reported in cytogenetic studies, confirming the accuracy of CGH in detecting genetic abnormalities in FL. 21% of samples displayed normal profiles, probably reflecting the absence of unbalanced abnormality, which is also in agreement with the cytogenetic data. In conclusion, we showed that CGH is an accurate, reliable and rapid method and we propose the inclusion of CGH in the evaluation of FL at diagnosis.
采用比较基因组杂交（CGH）技术分析了34例滤泡性淋巴瘤（FL）样本。27例样本显示至少一个基因组区域存在DNA序列拷贝数变化（26例样本至少有一处增益，9例样本至少有一处缺失）。某些染色体或染色体区域更易受累。最常出现增益的区域是18q染色体（占样本的29%）、X染色体（21%）、7号染色体（18%）、2号染色体、6p染色体和8q染色体（12%）。有两个区域更易出现缺失：6q染色体（12%）和17p染色体（9%）。所有这些出现增益和缺失的区域先前在细胞遗传学研究中均有报道，证实了CGH在检测FL基因异常方面的准确性。21%的样本显示正常图谱，这可能反映了不存在不平衡异常，这也与细胞遗传学数据相符。总之，我们表明CGH是一种准确、可靠且快速的方法，我们建议在FL诊断评估中纳入CGH。
