Boyano M D, Garcia de Galdeano A, Smith-Zubiaga I, Cañavate M L
Department of Cell Biology and Morphological Sciences, School of Medicine, University of the Basque Country, Leioa, Vizcaya, Spain.
Anticancer Res. 1997 Mar-Apr;17(2A):1135-41.
We had previously shown that murine B16F10 melanoma cells express the receptor for IL-2, transcribe the gene for IL-2 and respond to its factor by increasing their proliferation. In the present work we have investigated the effect of in vitro IL-2 treatment on the metastatic ability of B16F10 cells. In vivo experiments showed that the metastatic colonization of the liver was notably higher after intrasplenic inoculation of IL-2-treated cells. However, no change was observed when cells were intravenously inoculated. In vitro, cells became more resistant to NK lysis although the cytometric analysis of class 1 MHC molecules revealed a decrease in H-2Kb expression. In contrast IL-2 induced a two fold increment in the expression of la antigen. On the other hand slot-blot analysis showed that IL-2 gene expression could be upregulated, however no free IL-2 was released into the culture medium of B16F10 cells. We conclude that IL-2 increases the ability of B16F10 cells to metastase to the liver. The increase in the resistance to NK activity and in la antigen expression could be involved in the mechanisms underlying this effect.
我们先前已表明，小鼠B16F10黑色素瘤细胞表达白细胞介素-2（IL-2）受体，转录IL-2基因，并通过增加增殖对其因子作出反应。在本研究中，我们研究了体外IL-2处理对B16F10细胞转移能力的影响。体内实验表明，脾脏内接种经IL-2处理的细胞后，肝脏的转移定植明显更高。然而，静脉内接种细胞时未观察到变化。在体外，细胞对自然杀伤（NK）细胞裂解的抵抗力增强，尽管对1类主要组织相容性复合体（MHC）分子的细胞计数分析显示H-2Kb表达降低。相反，IL-2诱导Ia抗原表达增加两倍。另一方面，狭缝印迹分析表明IL-2基因表达可上调，但没有游离的IL-2释放到B16F10细胞的培养基中。我们得出结论，IL-2增加了B16F10细胞转移至肝脏的能力。对NK活性抵抗力的增加和Ia抗原表达的增加可能参与了这种效应的潜在机制。
