Boyano D, Smith-Zubiaga I, Alvarez A, Cantón I, Cañavate L
Department of Cell Biology and Morphological Sciences, School of Medicine and Dentistry, University of the Basque Country, Vizcaya, Spain.
Eur Cytokine Netw. 1998 Jun;9(2):187-92.
In previous studies, we demonstrated the presence of the interleukin-2 (IL-2) signalling system in B16F10 murine melanoma and observed that in vitro treatment of B16F10 cells with IL-2, enhanced metastasis. To further understand the role played by interleukins in melanoma, we examined the effect of IL-6 on the metastatic activity and properties of B16 melanoma cells. We observed that B16F10 cells, cultured in the presence of IL-6, showed a clear increase in their metastatic ability, both in the liver and in the lungs. Neither cell proliferation nor in vitro colony formation were affected by IL-6; however, the expression of CD44 and VLA-4 increased. The IL-6 gene was expressed in B16F10 cells as shown by RT-PCR. A slight induction of IL-6 mRNA expression by IL-2 was observed, but not after treatment with IL-1beta or IL-6. Nevertheless, no soluble IL-6 could be detected in cell supernatants even after treatment with IL-2. Finally, we tested the effect of IL-1beta, IL-6 and IL-2 on the expression of the IL-2 receptor (IL-2R). IL-1beta turned out to be the strongest inducer of IL-2R expression in B16F10 cells. Altogether, these data confirm the involvement of interleukins in the biology and metastatic activity of melanoma.
在先前的研究中，我们证实在B16F10小鼠黑色素瘤中存在白细胞介素-2（IL-2）信号系统，并观察到用IL-2体外处理B16F10细胞可增强转移能力。为了进一步了解白细胞介素在黑色素瘤中所起的作用，我们研究了IL-6对B16黑色素瘤细胞转移活性和特性的影响。我们观察到，在IL-6存在下培养的B16F10细胞，其在肝脏和肺部的转移能力均明显增强。IL-6对细胞增殖和体外集落形成均无影响；然而，CD44和VLA-4的表达增加。RT-PCR结果显示IL-6基因在B16F10细胞中表达。观察到IL-2可轻微诱导IL-6 mRNA表达，但IL-1β或IL-6处理后未出现这种情况。尽管如此，即使在用IL-2处理后，细胞上清液中也未检测到可溶性IL-6。最后我们检测了IL-1β、IL-6和IL-2对IL-2受体（IL-2R）表达的影响。结果表明IL-1β是B16F10细胞中IL-2R表达的最强诱导剂。总之，这些数据证实白细胞介素参与了黑色素瘤的生物学行为和转移活性。
