Graham E T
Department of Ornamental Horticulture, University of Tennessee, Knoxville 37901-1071, USA.
Biotech Histochem. 1997 May;72(3):119-22. doi: 10.3109/10520299709082223.
Immature fruit of tomato, Lycopersicon esculentum (Celebrity), was examined to observe the cuticle, its interface with the epidermis, and the general histology of the outer exocarp. Paraffin sections were stained first with Bismarck brown Y. Structures already stained in various hues of brown were stained again with either azure B, aluminum hematoxylin and alcian blue SGX, or the periodic acid-Schiff (PAS) reaction. Bismarck brown-azure B displayed the cuticle in strong contrast with subjacent tissue; however, nuclei were not easily identified at low magnification. Bismarck brown-hematoxylin-alcian blue produced a sharply contrasted combination of yellow cuticle, bright blue cell walls and purple nuclei. Nuclei stained purple with hematoxylin were easily identified at x100. Bismarck brown-PAS stained the cuticle golden brown and subjacent tissues mageta red. Surprisingly, epidermal cells stained specifically and intensely with PAS while pretreatment with an aldehyde blockade and omission of periodic acid prevented staining of all other tissues.
对番茄（Lycopersicon esculentum，品种为“名人”）的未成熟果实进行检查，以观察其角质层、角质层与表皮的界面以及外果皮的一般组织学结构。石蜡切片首先用俾斯麦棕Y染色。已被染成各种棕色色调的结构再用天青B、铝苏木精和阿尔辛蓝SGX或过碘酸-希夫（PAS）反应进行染色。俾斯麦棕-天青B染色使角质层与下方组织形成强烈对比；然而，在低倍镜下不容易识别细胞核。俾斯麦棕-苏木精-阿尔辛蓝产生了黄色角质层、亮蓝色细胞壁和紫色细胞核的鲜明对比组合。用苏木精染成紫色的细胞核在100倍放大倍数下很容易识别。俾斯麦棕-PAS染色使角质层呈金棕色，下方组织呈品红色。令人惊讶的是，表皮细胞经PAS特异性强烈染色，而用醛阻断剂预处理并省略过碘酸可防止所有其他组织染色。
