膜片钳记录中通道数量的估计：应用于电压门控通道多通道数据的动力学分析
Estimating the number of channels in patch-clamp recordings: application to kinetic analysis of multichannel data from voltage-operated channels.
作者信息
Baumgartner W, Hohenthanner K, Höfer G F, Groschner K, Romanin C
机构信息
Institute for Biophysics, University of Linz, Austria.
出版信息
Biophys J. 1997 Mar;72(3):1143-52. doi: 10.1016/S0006-3495(97)78763-0.
Abstract
Important kinetic information of voltage-operated ion channels can be obtained by estimating the open probability, the availability, and the first latency, and by applying run analysis. In the case of multichannel patches, estimation of the number of available channels is a prerequisite for the above analysis. Here we describe a method for calculation of the a posteriori probability of the number of available channels in each sweep by using the Bayes formula. This probability serves as a measure for the number of channels and allows for first latency determination and run analysis. The methods described were applied to simulated and experimental data obtained from L-type Ca2+ channel recordings.
摘要
通过估计开放概率、可用性和首次潜伏期，并应用运行分析，可以获得电压门控离子通道的重要动力学信息。对于多通道膜片，估计可用通道的数量是上述分析的前提条件。在此，我们描述一种使用贝叶斯公式计算每次扫描中可用通道数量的后验概率的方法。该概率可作为通道数量的一种度量，并允许进行首次潜伏期测定和运行分析。所描述的方法应用于从L型Ca2+通道记录获得的模拟和实验数据。
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