一种用于定量检测人胎儿血红蛋白的改良色谱方法。

An improved chromatographic procedure for quantitation of human fetal hemoglobin.

作者信息

Abraham E C, Reese A, Stallings M, Garver F A, Huisman T H

出版信息

Hemoglobin. 1977;1(6):547-60. doi: 10.3109/03630267709003419.

Abstract

The DEAE-cellulose chromatographic procedure which uses glycine-KCN-NaCl solutions as Developers and a NaCl gradient to elute the hemoglobin zones was found to be useful for the quantitation of Hb F in samples containing Hb A, provided the level of Hb F exceeds 2 percent. The method has been evaluted through a study of artificial mixtures and of blood samples from patients with various disorders. The data have been compared with results obtained by four other procedures that are used for the quantitation of Hb F.

摘要

使用甘氨酸 - 氰化钾 - 氯化钠溶液作为展开剂并采用氯化钠梯度洗脱血红蛋白区带的二乙氨基乙基纤维素色谱法，被发现对于定量含有血红蛋白A的样品中的血红蛋白F很有用，前提是血红蛋白F的水平超过2%。该方法已通过对人工混合物和患有各种疾病患者的血样研究进行了评估。已将这些数据与通过用于定量血红蛋白F的其他四种方法获得的结果进行了比较。