镰状细胞病患者胎儿血红蛋白的微量色谱定量分析。

Microchromatographic quantitation of fetal hemoglobin in patients with sickle cell disease.

作者信息

Abraham E C, Carver J, Döbler J, Milner P F, Huisman T H

出版信息

Hemoglobin. 1979;3(5):341-51. doi: 10.3109/03630267908997539.

Abstract

A microchromatographic procedure (Isolab Fast Hb Test System) which was developed for the quantitation of Hb AI (10) has been found useful for the quantitation of Hb F in samples that contain Hb S and/or Hb C but no Hb A (% Fmicro). This method has been evaluated through analyses of known mixtures of Hb F and Hb S. The Hb Fmicro levels in patients with sickle cell anemia and related conditions were compared with results obtained by alkali denaturation (% FAD) and conventional DEAE-cellulose chromatography (% FDE). This microchromatographic technique is a fast, simple, and sensitive method for Hb F quantitation in patients with Sickle cell disease.

摘要

一种为定量检测Hb AI（10）而开发的微量色谱法（Isolab快速血红蛋白检测系统），已被证明可用于定量检测含有Hb S和/或Hb C但不含Hb A的样本中的Hb F（% Fmicro）。通过分析已知的Hb F和Hb S混合物对该方法进行了评估。将镰状细胞贫血及相关病症患者的Hb Fmicro水平与通过碱变性法（% FAD）和传统二乙氨基乙基纤维素色谱法（% FDE）获得的结果进行了比较。这种微量色谱技术是一种用于镰状细胞病患者Hb F定量检测的快速、简便且灵敏的方法。