显微注射冻干精子核后哺乳动物卵子的原核形成与卵裂

Pronuclear formation and cleavage of mammalian eggs after microsurgical injection of freeze-dried sperm nuclei.

作者信息

Hoshi K, Yanagida K, Katayose H, Yazawa H

机构信息

Fukushima Medical College, Japan.

出版信息

Zygote. 1994 Aug;2(3):237-42. doi: 10.1017/s0967199400002033.

DOI:10.1017/s0967199400002033

PMID:8785682

Abstract

A study was conducted to evaluate the ability of mammalian oocytes to develop to the pronuclear stage and beyond if injected with freeze-dried sperm nuclei. The rate of development of hamster eggs to the pronuclear stage after microinjection of freeze-dried hamster sperm nuclei was 90%. The pronuclear formation rate of hamster eggs injected with freeze-dried human sperm nuclei was 85%. On the other hand, the rates for eggs injected with untreated sperm nuclei were 84% and 89% respectively. The survival rate of rabbit eggs microinjected with freeze-dried rabbit sperm nuclei was 64%, the fertilisation rate 56% and the cleavage rate 38%. The survival, fertilisation and cleavage rates of eggs injected with non-freeze-dried sperm nuclei were 78%, 45% and 34%, respectively. There was no difference between eggs injected with freeze-dried sperm nuclei and those injected with sperm nuclei that had not been freeze-dried. Of the viable rabbit eggs injected with freeze-dried rabbit sperm nuclei, 6% developed to the 6- to 8-cell stage 62 h after injection.

摘要

开展了一项研究，以评估如果向哺乳动物卵母细胞注射冻干的精子细胞核，其发育至原核阶段及以后阶段的能力。显微注射冻干的仓鼠精子细胞核后，仓鼠卵发育至原核阶段的比率为90%。注射冻干的人类精子细胞核的仓鼠卵的原核形成率为85%。另一方面，注射未处理精子细胞核的卵的比率分别为84%和89%。显微注射冻干的兔精子细胞核的兔卵的存活率为64%，受精率为56%，卵裂率为38%。注射未冻干精子细胞核的卵的存活、受精和卵裂率分别为78%、45%和34%。注射冻干精子细胞核的卵与注射未冻干精子细胞核的卵之间没有差异。在注射冻干兔精子细胞核的存活兔卵中，6%在注射后62小时发育至6至8细胞阶段。