Yabe N, Matsui H
Department of Hygiene, Dokkyo University School of Medicine, Tochigi, Japan.
J Ethnopharmacol. 1997 Mar;56(1):67-76. doi: 10.1016/s0378-8741(96)01508-5.
We describe the effects of an ethanol-extracted fraction of berries of Ampelopsis brevipedunculata (Maxim.) Trautv. (Vitaceae), a plant used in folk medicine to treat liver disease, on the synthesis of non-collagenous proteins and collagen by rat collagen-producible cells such as dermal fibroblasts and liver non-parenchymal Ito cells. The generation of superoxide and hydroxyl radical was assessed by measuring the reduction of cytochrome c and the formation of thiobarbituric acid-reactive substances from deoxyribose, respectively. The synthesis of non-collagenous proteins and collagen as evaluated by measuring the extent of [3H]tryptophan incorporation into a total protein fraction of culture products and the [3H]proline-incorporating rate into a collagenase-digestible protein fraction, respectively. Both types of cells promptly synthesized only collagen in response to a dialyzable fraction of the extract. Major activity to generate oxygen free radicals accumulated in the dialyzable fraction whereas activity to decrease ferrous iron-mediated generation of the radicals accumulated in an undialyzable fraction of the extract. Stimulation of collagen synthesis was caused by superoxide because addition of superoxide dismutase but not pyruvate, an antioxidant of hydrogen peroxide, or dimethyl sulfoxide, an antioxidant of the hydroxyl radical, abrogated the stimulatory effect. The extract may arrest the progress of liver injury mediated by oxygen free radicals generated in the presence of ferrous iron.
我们描述了蛇葡萄（Ampelopsis brevipedunculata (Maxim.) Trautv.，葡萄科）果实的乙醇提取物对大鼠可产生胶原蛋白的细胞（如真皮成纤维细胞和肝脏非实质细胞——伊托细胞）合成非胶原蛋白和胶原蛋白的影响。蛇葡萄是一种民间用于治疗肝病的植物。通过分别测量细胞色素c的还原以及由脱氧核糖生成硫代巴比妥酸反应性物质来评估超氧阴离子和羟基自由基的生成。通过分别测量[³H]色氨酸掺入培养产物总蛋白组分的程度以及[³H]脯氨酸掺入胶原酶可消化蛋白组分的速率，来评估非胶原蛋白和胶原蛋白的合成。两种类型的细胞对提取物的可透析组分迅速做出反应，仅合成胶原蛋白。产生氧自由基的主要活性积聚在可透析组分中，而降低亚铁离子介导的自由基生成的活性则积聚在提取物的不可透析组分中。超氧阴离子导致胶原蛋白合成受到刺激，因为添加超氧化物歧化酶可消除刺激作用，而添加过氧化氢的抗氧化剂丙酮酸或羟基自由基的抗氧化剂二甲基亚砜则不能。该提取物可能会阻止在亚铁离子存在下产生的氧自由基介导的肝损伤进程。
