Yabe N, Tanaka K, Matsui H
Department of Hygiene, Dokkyo University School of Medicine, Mibu, Tochigi, Japan.
J Ethnopharmacol. 1998 Jan;59(3):147-59. doi: 10.1016/s0378-8741(97)00121-9.
We characterized the effects of an ethanol-extract of the berries of Ampelopsis brevipedunculata (Maxim.) Trautv. (Vitaceae), a plant used in folk medicine to treat liver disease, on rat hepatocyte injury occurring spontaneously, stimulated with ferrous iron and with xanthine oxidase in combination with hypoxanthine or stimulated with ethanol in serum-free culture. Total intracellular and extracellular activities of lactate dehydrogenase (LDH) accumulating during incubation and the percentage of intracellular LDH activity released into culture medium were routinely measured, to evaluate the degree of the injury. The extract decreased a high level of LDH release spontaneously occurring and an elevated level of LDH release stimulated with ferrous iron to approximately the level caused by antioxidants, such as superoxide dismutase, pyruvate and dimethyl sulfoxide. Xanthine oxidase-stimulated LDH release was not decreased by the extract. Ethanol-stimulated LDH release was decreased by the extract when the spontaneous release level was comparatively high. These results indicate that the extract inhibits intact hepatocytes from degrading, by the toxic effect of iron released from primary injured hepatocytes through the generation of reactive oxygen species. The major antitoxic activity of the extract was found in an undialyzable fraction. Sugars were necessary to exert the activity as estimated by periodate oxidation of the extract.
我们对民间用于治疗肝病的植物蛇葡萄（葡萄科）果实的乙醇提取物，在无血清培养中对大鼠肝细胞自发损伤、亚铁离子刺激损伤、黄嘌呤氧化酶与次黄嘌呤联合刺激损伤以及乙醇刺激损伤的作用进行了表征。在孵育过程中，常规测量细胞内和细胞外乳酸脱氢酶（LDH）的总活性以及释放到培养基中的细胞内LDH活性百分比，以评估损伤程度。该提取物可将自发产生的高水平LDH释放以及亚铁离子刺激产生的升高的LDH释放水平降低至抗氧化剂（如超氧化物歧化酶、丙酮酸和二甲基亚砜）所导致的水平左右。黄嘌呤氧化酶刺激的LDH释放未被该提取物降低。当自发释放水平相对较高时，乙醇刺激的LDH释放可被该提取物降低。这些结果表明，该提取物通过从原发性损伤肝细胞释放的铁经活性氧生成的毒性作用，抑制完整肝细胞的降解。提取物的主要抗毒活性存在于不可透析部分。通过提取物的高碘酸盐氧化估计，糖类对于发挥该活性是必需的。
