唾液中的抗HIV抗体：唾液成分、检测方法及采集系统作用的评估

Anti-HIV antibody in saliva: an assessment of the role of the components of saliva, testing methodologies and collection systems.

作者信息

Lamey P J, Nolan A, Follett E A, Coote I, MacFarlane T W, Kennedy D H, Connell A, Parry J V

机构信息

The Queen's University of Belfast, N.Ireland.

出版信息

J Oral Pathol Med. 1996 Mar;25(3):104-7. doi: 10.1111/j.1600-0714.1996.tb00202.x.

DOI:10.1111/j.1600-0714.1996.tb00202.x

PMID:9148035

Abstract

The various components of saliva, namely mixed saliva, parotid saliva, submandibular saliva, crevicular fluid and minor (labial) gland secretions, were collected from 63 known HIV antibody seropositive patients. A commercial test system, Wellcozyme HIV 1+2, and an antibody capture ELISA (GACELISA), were compared for sensitivity against all components. Sensitivity of the GACELISA system was 100% in 123 mixed saliva, 121 parotid saliva and 127 labial fluid samples, and 98% in 99 submandibular samples and 127 crevicular fluid samples. Respective figures for Wellcozyme 1+2 were 92%, 55%, 73%, 66% and 63%. Mixed saliva was most easily, conveniently and effectively collected using a plain Salivette. In 241 Salivette samples examined from the 63 patients, GACELISA proved 100% sensitive, and Wellcozyme 95% sensitive. Another form of Salivette impregnated with citric acid was unsuitable for GACELISA and gave a false negative value of 45%. In 197 samples from the gingival margin taken by a dry swab, GACELISA showed a sensitivity of 98% and Wellcozyme 81%. The most sensitive method for demonstrating anti-HIV antibody in saliva is to collect mixed saliva with the plain Salivette system and assay anti-HIV antibody levels by GACELISA.

摘要

从63名已知HIV抗体血清阳性患者中收集了唾液的各种成分，即混合唾液、腮腺唾液、颌下唾液、龈沟液和小（唇）腺分泌物。将一种商业检测系统Wellcozyme HIV 1+2和一种抗体捕获ELISA（GACELISA）针对所有成分的敏感性进行了比较。GACELISA系统在123份混合唾液、121份腮腺唾液和127份唇腺液样本中的敏感性为100%，在99份颌下样本和127份龈沟液样本中的敏感性为98%。Wellcozyme 1+2的相应数据分别为92%、55%、73%、66%和63%。使用普通唾液采集管最容易、方便且有效地收集混合唾液。在对63名患者的241份唾液采集管样本进行检测时，GACELISA的敏感性为100%，Wellcozyme为95%。另一种浸渍柠檬酸的唾液采集管形式不适用于GACELISA，其假阴性值为45%。在通过干拭子从牙龈边缘采集的197份样本中，GACELISA的敏感性为98%，Wellcozyme为81%。在唾液中检测抗HIV抗体最敏感的方法是使用普通唾液采集管系统收集混合唾液，并通过GACELISA检测抗HIV抗体水平。