Stephenson J R, Reynolds R K, Devare S G, Reynolds F H
J Biol Chem. 1977 Nov 10;252(21):7818-25.
The major nonglycosylated structure proteins of mammalian type C RNA tumor viruses are synthesized in the form of a high molecular weight precursor coded for by a viral gene disignated "gag". Previous genetic analysis of a prototype virus isolated of mouse origin has led to a determination of the internal arragnement of the regions within the gag gene coding for individual structural proteins. In the present study, the biochemical properties of structural proteins of type C virus isolates of additional mammalian species were analyzed. The results obtained indicate that the biochemical properties of immunologically cross-reactive proteins have been highly conserved throughout the evolution of this group of viruses. Moreover, these findings provide a means of mapping the gag genes of a broad range of mammalian type C viruses. In view of the results obtained, a new nomenclature system for type C viral gag gene-coded translational products is proposed.
哺乳动物C型RNA肿瘤病毒的主要非糖基化结构蛋白是以一种高分子量前体的形式合成的，该前体由一个名为“gag”的病毒基因编码。先前对一种源自小鼠的原型病毒进行的遗传分析，已确定了gag基因内编码各个结构蛋白的区域的内部排列。在本研究中，分析了其他哺乳动物物种的C型病毒分离株的结构蛋白的生化特性。所得结果表明，在这组病毒的整个进化过程中，免疫交叉反应蛋白的生化特性高度保守。此外，这些发现提供了一种绘制多种哺乳动物C型病毒gag基因图谱的方法。鉴于所得结果，提出了一种C型病毒gag基因编码的翻译产物的新命名系统。
