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2
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NEW DEVELOPMENTS IN THE GOLGI CONTROVERSY.
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The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
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Nucleosidediphosphatase activity in the Golgi apparatus and its usefulness for cytological studies.高尔基体中的核苷二磷酸酶活性及其在细胞学研究中的用途。
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The histochemical demonstration of secretory capillaries in the pancreas with the aid of substrate specific phosphatases.借助底物特异性磷酸酶对胰腺中分泌性毛细血管进行组织化学显示。
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Intracisternal granules in the exocrine cells of the pancreas.胰腺外分泌细胞中的脑池内颗粒。
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Structural modulations of plasmalemmal vesicles.质膜囊泡的结构调节
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Intracellular transport of secretory proteins in the pancreatic exocrine cell. II. Transport to condensing vacuoles and zymogen granules.胰腺外分泌细胞中分泌蛋白的细胞内运输。II. 向浓缩泡和酶原颗粒的运输。
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Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex.胰腺外分泌细胞中分泌蛋白的细胞内运输。I. 高尔基体复合体周边元件的作用。
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The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.注入的辣根过氧化物酶在小鼠肾近端小管吸收的早期阶段：一种新技术的超微结构细胞化学研究
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Fenestrated cisternae in the Golgi apparatus of the epididymis.附睾高尔基体中的有孔池。
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哺乳动物外分泌胰腺分泌过程的研究。I. 浓缩泡。

Studies of the secretory process in the mammalian exocrine pancreas. I. The condensing vacuoles.

作者信息

Novikoff A B, Mori M, Quintana N, Yam A

出版信息

J Cell Biol. 1977 Oct;75(1):148-65. doi: 10.1083/jcb.75.1.148.

DOI:10.1083/jcb.75.1.148
PMID:914894
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2111565/
Abstract

Phosphatase cytochemistry was used to distinguish between the Golgi apparatus and GERL (considered as a specialized region of endoplasmic reticulum [ER] at the inner [trans] aspect of the Golgi stack) in pancreatic exocrine cells of guinea pig, rat, rabbit, and hamster. The trans element of the Golgi stack exhibits thiamine pyrophosphatase (TPPase) but no acid phosphatase (AcPase) activity. In contrast, GERL shows AcPase but no TPPase activity. The nascent secretory granules, or condensing vacuoles, are expanded cisternal portions of GERL. Continuities of condensing vacuoles with rough ER are suggested, and it is proposed that some secretory components may have direct access to the condensing vacuoles from ER. Connections of Golgi apparatus with GERL were not seen.

摘要

利用磷酸酶细胞化学方法，在豚鼠、大鼠、兔和仓鼠的胰腺外分泌细胞中区分高尔基体和GERL（被认为是高尔基体堆栈内侧[反面]内质网[ER]的一个特殊区域）。高尔基体堆栈的反面成分表现出硫胺素焦磷酸酶（TPPase）活性，但没有酸性磷酸酶（AcPase）活性。相反，GERL显示出AcPase活性，但没有TPPase活性。新生分泌颗粒或浓缩泡是GERL扩张的潴泡部分。提示浓缩泡与粗面内质网有连续性，并且有人提出一些分泌成分可能直接从内质网进入浓缩泡。未观察到高尔基体与GERL之间的连接。