Novikoff A B, Mori M, Quintana N, Yam A
J Cell Biol. 1977 Oct;75(1):148-65. doi: 10.1083/jcb.75.1.148.
Phosphatase cytochemistry was used to distinguish between the Golgi apparatus and GERL (considered as a specialized region of endoplasmic reticulum [ER] at the inner [trans] aspect of the Golgi stack) in pancreatic exocrine cells of guinea pig, rat, rabbit, and hamster. The trans element of the Golgi stack exhibits thiamine pyrophosphatase (TPPase) but no acid phosphatase (AcPase) activity. In contrast, GERL shows AcPase but no TPPase activity. The nascent secretory granules, or condensing vacuoles, are expanded cisternal portions of GERL. Continuities of condensing vacuoles with rough ER are suggested, and it is proposed that some secretory components may have direct access to the condensing vacuoles from ER. Connections of Golgi apparatus with GERL were not seen.
利用磷酸酶细胞化学方法,在豚鼠、大鼠、兔和仓鼠的胰腺外分泌细胞中区分高尔基体和GERL(被认为是高尔基体堆栈内侧[反面]内质网[ER]的一个特殊区域)。高尔基体堆栈的反面成分表现出硫胺素焦磷酸酶(TPPase)活性,但没有酸性磷酸酶(AcPase)活性。相反,GERL显示出AcPase活性,但没有TPPase活性。新生分泌颗粒或浓缩泡是GERL扩张的潴泡部分。提示浓缩泡与粗面内质网有连续性,并且有人提出一些分泌成分可能直接从内质网进入浓缩泡。未观察到高尔基体与GERL之间的连接。