Lentz T L, Chester J
J Cell Biol. 1977 Oct;75(1):258-67. doi: 10.1083/jcb.75.1.258.
The localization of cholinergic receptors in brain synaptosomes and in synapses of the midbrain reticular formation and hypothalamic preoptic nucleus has been demonstrated by means of a horseradish peroxidase-alpha-bungarotoxin (HRP-alpha-Btx) conjugate. Only a small proportion of the total number of synapses was reactive. Axon terminals of reactive synapses contained primarily small clear vesicles, while synapses characterized by large numbers of dense core vesicles were unreactive. Toxin-binding sites were found to occur in a thickened zone of the postsynaptic surface. This procedure can be employed to study the regional distribution and localization of nicotinic receptor sites in the central nervous system.
通过辣根过氧化物酶-α-银环蛇毒素（HRP-α-Btx）偶联物已证实胆碱能受体在脑突触体以及中脑网状结构和下丘脑视前核突触中的定位。在突触总数中只有一小部分有反应。有反应的突触的轴突终末主要含有小而清亮的囊泡，而以大量致密核心囊泡为特征的突触则无反应。毒素结合位点见于突触后表面的增厚区域。该方法可用于研究中枢神经系统中烟碱样受体位点的区域分布和定位。
