Deguchi M, Tomioka Y, Mizugaki M, Tagami H
Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan.
Dermatology. 1998;196(3):292-4. doi: 10.1159/000017923.
Data on the association of human papillomavirus (HPV) infection with extragenital Bowen's disease are very scarce.
To evaluate the prevalence of HPV infection in extragenital Bowen's disease showing histologically a number of koilocytes in the lesional epidermis, we studied formalin-fixed, paraffin-embedded tissues of 9 such cases.
HPV DNA was studied in such samples by the polymerase chain reaction (PCR) and in situ hybridization.
Despite negative results with in situ hybridization, the PCR with HPV type 33 primer detected the presence of virus DNA in 2 out of 9 cases.
As far as we know, this is the first report of the detection of HPV type 33 DNA in the lesional skin of extragenital Bowen's disease. The prevalence of HPV infection in extragenital Bowen's disease may be higher than expected, especially in cases histologically showing many koilocytes in the lesional epidermis.
关于人乳头瘤病毒（HPV）感染与生殖器外鲍温病相关性的数据非常稀少。
为评估在组织学上显示病变表皮中有多个凹空细胞的生殖器外鲍温病中HPV感染的患病率，我们研究了9例此类病例的福尔马林固定、石蜡包埋组织。
通过聚合酶链反应（PCR）和原位杂交对此类样本中的HPV DNA进行研究。
尽管原位杂交结果为阴性，但使用33型HPV引物进行的PCR在9例中的2例中检测到了病毒DNA的存在。
据我们所知，这是首次在生殖器外鲍温病病变皮肤中检测到33型HPV DNA的报告。生殖器外鲍温病中HPV感染的患病率可能高于预期，尤其是在组织学上显示病变表皮中有许多凹空细胞的病例中。
