从山羊初乳中获得的单唾液酸三糖的结构测定
Structural determination of monosialyl trisaccharides obtained from caprine colostrum.
作者信息
Urashima T, Murata S, Nakamura T
机构信息
Department of Bioresource Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
出版信息
Comp Biochem Physiol B Biochem Mol Biol. 1997 Apr;116(4):431-5. doi: 10.1016/s0305-0491(96)00269-6.
Acidic oligosaccharides were separated by dialysis, ion-exchange, preparative paper and gel chromatography from caprine colostrum. Four sialyl trisaccharides were characterized by 1H-NMR spectrometry as follows: alpha-N-acetylneuraminyl-(2,6)-beta-D-galactopyranosyl-(1,4)-2-N-acetami do-2 -deoxy-D-glucopyranose (Neu5Ac alpha 2-6Gal beta 1-4GlcNAc), alpha-N-acetylneuraminyl-(2,3)-beta-D-galactopyranosyl-(1,4)-D-glucopyra nose (Neu5Ac alpha 2-3Gal beta-1-4Glc), alpha-N-acetylneuraminyl-(2,6)-beta-D-galactopyranosyl-(1,4)-D-glucopyra nose (Neu5Ac alpha 2-6Gal beta 1-4Glc) and alpha-N-glycolylneuraminyl-(2,6)-beta-D-galactopyranosyl-(1,4)-D -glucopyranose (Neu5Gc alpha 2-6Gal beta 1-4Glc).
通过透析、离子交换、制备纸色谱法和凝胶色谱法从山羊初乳中分离出酸性寡糖。通过1H-NMR光谱对四种唾液酸三糖进行了表征，具体如下：α-N-乙酰神经氨酸-(2,6)-β-D-吡喃半乳糖基-(1,4)-2-N-乙酰氨基-2-脱氧-D-吡喃葡萄糖（Neu5Acα2-6Galβ1-4GlcNAc）、α-N-乙酰神经氨酸-(2,3)-β-D-吡喃半乳糖基-(1,4)-D-吡喃葡萄糖（Neu5Acα2-3Galβ-1-4Glc）、α-N-乙酰神经氨酸-(2,6)-β-D-吡喃半乳糖基-(1,4)-D-吡喃葡萄糖（Neu5Acα2-6Galβ1-4Glc）和α-N-羟乙酰神经氨酸-(2,6)-β-D-吡喃半乳糖基-(1,4)-D-吡喃葡萄糖（Neu5Gcα2-6Galβ1-4Glc）。
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