使用红外荧光检测对带有性别等位基因的STR基因座进行多重扩增。
Multiplex amplification of STR loci with gender alleles using infrared fluorescence detection.
作者信息
Steffens D L, Roy R, Brumbaugh J A
机构信息
LI-COR, Inc., Biotechnology Division, Lincoln, NE 68504, USA.
出版信息
Forensic Sci Int. 1997 Mar 14;85(3):225-32. doi: 10.1016/s0379-0738(97)02104-x.
The analysis of short tandem repeat (STR) polymorphisms has proven extremely useful for gene mapping, paternity testing, and forensic analysis. Several commercial products are currently available for performing amplification and analysis of STRs. We have adapted Promega Geneprint Systems for use with a high sensitivity infrared (IR) fluorescent automated DNA sequencer. IR-labeled amplification products are generated by including a small quantity of IR-labeled dATP in the reaction. Several Geneprint STR loci can be multiplexed together with the amelogenin sex identification locus in a single amplification reaction. We have successfully amplified up to five Geneprint STR loci together with the amelogenin locus thus improving the throughput of analysis. Purified genomic DNA as well as simulated forensic samples have been utilized for these multiplex amplifications.
短串联重复序列（STR）多态性分析已被证明在基因定位、亲子鉴定和法医分析中极为有用。目前有几种商业产品可用于进行STR的扩增和分析。我们已对Promega基因指纹系统进行了改造，使其适用于高灵敏度红外（IR）荧光自动DNA测序仪。通过在反应中加入少量IR标记的dATP来生成IR标记的扩增产物。几个基因指纹STR位点可以与牙釉蛋白性别鉴定位点在单个扩增反应中进行多重扩增。我们已成功地将多达五个基因指纹STR位点与牙釉蛋白位点一起进行扩增，从而提高了分析通量。纯化的基因组DNA以及模拟法医样本已用于这些多重扩增。
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