Peredo H A, Feleder E C, Adler-Graschinsky E
Instituto de Investigaciones Farmacológicas (ININFA, CONICET), Buenos Aires, Argentina.
Prostaglandins Leukot Essent Fatty Acids. 1997 Apr;56(4):253-8. doi: 10.1016/s0952-3278(97)90567-6.
The roles of nitric oxide and of endothelium in the effects of the vasorelaxing agents acetylcholine and bradykinin on the production of prostanoids was studied in the isolated and perfused mesenteric vascular bed of the rat. Prostanoids were measured in the perfusate by high-performance liquid chromatography (HPLC). In the intact vascular bed, 1 microM bradykinin increased the release of 6-keto-prostaglandinF(1alpha) (stable metabolite of prostacyclin) and of prostaglandin E2 and 10 microM acetylcholine stimulated the efflux of prostacyclin only. In the de-endothelialized vascular bed, bradykinin increased the release of prostacyclin whereas acetylcholine increased the efflux of thromboxane. The inhibition of nitric oxide synthesis with 100 microM N(G)-nitro-L-arginine methyl ester prevented the effect of bradykinin but did not modify the effects of acetylcholine on prostanoid release. In addition, 100 microM L-arginine reversed the inhibitory effect of N(G)-nitro L-arginine methyl ester on bradykinin-stimulated prostaglandin production. It is concluded that acetylcholine and bradykinin stimulate prostanoid release in the rat mesenteric vascular bed with different patterns and through different mechanisms.
在大鼠离体灌注肠系膜血管床中，研究了一氧化氮和内皮在血管舒张剂乙酰胆碱和缓激肽对前列腺素生成的影响中的作用。通过高效液相色谱法（HPLC）测定灌注液中的前列腺素。在完整的血管床中，1微摩尔缓激肽增加了6-酮-前列腺素F（1α）（前列环素的稳定代谢产物）和前列腺素E2的释放，而10微摩尔乙酰胆碱仅刺激了前列环素的流出。在去内皮的血管床中，缓激肽增加了前列环素的释放，而乙酰胆碱增加了血栓素的流出。用100微摩尔N（G）-硝基-L-精氨酸甲酯抑制一氧化氮合成可阻止缓激肽的作用，但不改变乙酰胆碱对前列腺素释放的影响。此外，100微摩尔L-精氨酸可逆转N（G）-硝基-L-精氨酸甲酯对缓激肽刺激的前列腺素生成的抑制作用。结论是，乙酰胆碱和缓激肽以不同模式并通过不同机制刺激大鼠肠系膜血管床中前列腺素的释放。
