Robbiano L, Mereto E, Migliazzi Morando A, Pastore P, Brambilla G
Institute of Pharmacology, University of Genoa, Italy.
Mutat Res. 1997 Apr 24;390(1-2):51-7. doi: 10.1016/s0165-1218(96)00165-6.
A micronucleus assay in vivo has been developed that is based on the use of freshly isolated kidney cells from mononephrectomized rats. In this validation study, a statistically significant increase in the frequency of micronucleated cells was detected in rats given i.p. a single dose of four kidney carcinogens, N-nitrosodimethylamine, N-nitrosodiethylamine, N-ethyl-N-hydroxyethylnitrosamine and N-nitroso-N-methylurea. The clastogenic effect was more marked when the same dose was injected for 3 successive days. As compared to controls, treated rats displayed a reduction in the frequency of binucleated cells, presumably due to a toxicity-induced inhibition of cellular proliferation. The proposed method should be suitable for the detection of the clastogenic effect of procarcinogens biotransformed into reactive species in the kidney.
已经开发出一种基于使用来自单侧肾切除大鼠的新鲜分离肾细胞的体内微核试验。在这项验证研究中，给大鼠腹腔注射单剂量的四种肾致癌物，即N-亚硝基二甲胺、N-亚硝基二乙胺、N-乙基-N-羟乙基亚硝胺和N-亚硝基-N-甲基脲后，检测到微核细胞频率有统计学意义的增加。当连续3天注射相同剂量时，致断裂效应更为明显。与对照组相比，处理后的大鼠双核细胞频率降低，推测是由于毒性诱导的细胞增殖抑制所致。所提出的方法应适用于检测在肾脏中生物转化为反应性物种的前致癌物的致断裂效应。
