de Girolamo P, Arcamone N, Rosica A, Gargiulo G
Department of Structures, Functions and Biological Technologies, University of Naples, Federico II, Italy.
Acta Histochem. 1997 Mar;99(1):13-22. doi: 10.1016/S0065-1281(97)80003-7.
The localization of vasoactive intestinal polypeptide (VIP)-immunoreactive (ir) nerve cell bodies and fibers has been studied in the gill arches of goldfish (Carassius auratus, L.) using the peroxidase-antiperoxidase (PAP) immunohistochemical method. It was found that VIP-ir nerve cell bodies are localized in connective tissue on the oral side of the gill arch; these cells were present as single cells, in couples or as small clusters. Moreover, a dense network of VIP-ir fibers was observed beneath the lining epithelium of the raker cushion. The possible involvement of this peptide in mucus secretion in the gill arches of teleost is discussed.
采用过氧化物酶-抗过氧化物酶(PAP)免疫组织化学方法,研究了金鱼(Carassius auratus, L.)鳃弓中血管活性肠肽(VIP)免疫反应性(ir)神经细胞体和纤维的定位。结果发现,VIP-ir神经细胞体位于鳃弓口腔侧的结缔组织中;这些细胞以单个细胞、成对或小簇的形式存在。此外,在鳃耙垫的内衬上皮下方观察到密集的VIP-ir纤维网络。本文讨论了该肽在硬骨鱼鳃弓黏液分泌中可能的作用。