Durmaz B, Marol S, Durmaz R, Oram O, Hepsen I F, Günal S
Department of Clinical Microbiology, Faculty of Medicine, Inonu University, Malatya, Turkey.
Arzneimittelforschung. 1997 Apr;47(4):413-5.
The purpose of this study was to determine the aqueous humor concentrations of topically applied ciprofloxacin (CAS 86393-32-0), ofloxacin (CAS 82419-36-1) and tobramycin (CAS 79645-27-5). Thirty patients undergoing cataract extraction or trabeculectomy were randomly divided into three groups and each of the group received either 0.3% ciprofloxacin, ofloxacin or tobramycin topical drops preoperatively. Eyedrops were instilled for six times at a frequency of one drop every 15 minutes, beginning 90 minutes before initiation of the surgery. At the time of surgery, 0.1 ml aqueous fluid was aspirated from the anterior chamber. Concentrations of the antimicrobial agents were determined using the microbroth dilution procedure outlined by the National Committee for Clinical Laboratory Standards. Escherichia coli (ATCC 25922) was used as a standard strain for determination of minimal inhibitory concentrations (MICs). The mean aqueous levels of ciprofloxacin and ofloxacin were found to be 0.092 +/- 0.077 microgram/ml, 0.964 +/- 0.693 microgram/ml, respectively. Tobramycin did not reach the concentration that could be detected by applied method.
The mean aqueous humor levels of ofloxacin and ciprofloxacin were more than the MICs levels for most of the ocular pathogens which may cause postoperative endophthalmitis.
本研究的目的是测定局部应用环丙沙星（CAS 86393-32-0）、氧氟沙星（CAS 82419-36-1）和妥布霉素（CAS 79645-27-5）在房水中的浓度。30例接受白内障摘除术或小梁切除术的患者被随机分为三组，每组术前分别接受0.3%环丙沙星、氧氟沙星或妥布霉素局部滴眼液。从手术开始前90分钟起，每隔15分钟滴一滴眼药水，共滴6次。手术时，从前房抽取0.1 ml房水。使用美国国家临床实验室标准委员会概述的微量肉汤稀释法测定抗菌药物的浓度。大肠埃希菌（ATCC 25922）用作测定最低抑菌浓度（MIC）的标准菌株。发现环丙沙星和氧氟沙星的平均房水浓度分别为0.092±0.077微克/毫升、0.964±0.693微克/毫升。妥布霉素未达到应用方法可检测的浓度。
氧氟沙星和环丙沙星的平均房水浓度高于大多数可能引起术后眼内炎的眼部病原体的MIC水平。
