Bridoux F, Badou A, Saoudi A, Bernard I, Druet E, Pasquier R, Druet P, Pelletier L
Institut National de la Sante et de la Recherche Medicale U28, Institut Fédératif de Recherche 30, Université Paul Sabatier, 31059 Toulouse, France.
J Exp Med. 1997 May 19;185(10):1769-75. doi: 10.1084/jem.185.10.1769.
Autoreactive anti-MHC class II T cells are found in Brown Norway (BN) and Lewis (LEW) rats that receive either HgCl2 or gold salts. These T cells have a T helper cell 2 (Th2) phenotype in the former strain and are responsible for Th2-mediated autoimmunity. In contrast, T cells that expand in LEW rats produce IL-2 and prevent experimental autoimmune encephalomyelitis, a cell-mediated autoimmune disease. The aim of this work was to investigate, using T cell lines derived from HgCl2-injected LEW rats (LEWHg), the effect of these autoreactive T cells on the development of Th2-mediated autoimmunity. The five LEWHg T cell lines obtained protect against Th2-mediated autoimmunity induced by HgCl2 in (LEW x BN)F1 hybrids. The lines produce, in addition to IL-2, IFN-gamma and TGF-beta, and the protective effect is TGF-beta dependent since protection is abrogated by anti-TGF-beta treatment. These results identify regulatory, TGF-beta-producing, autoreactive T cells that are distinct from classical Th1 or Th2 and inhibit both Th1- and Th2-mediated autoimmune diseases.
在接受氯化汞或金盐的棕色挪威（BN）大鼠和刘易斯（LEW）大鼠中发现了自身反应性抗II类主要组织相容性复合体（MHC）T细胞。在前一种品系中，这些T细胞具有辅助性T细胞2（Th2）表型，并负责Th2介导的自身免疫。相比之下，在LEW大鼠中扩增的T细胞产生白细胞介素-2（IL-2）并预防实验性自身免疫性脑脊髓炎，这是一种细胞介导的自身免疫性疾病。这项工作的目的是使用源自注射氯化汞的LEW大鼠（LEWHg）的T细胞系，研究这些自身反应性T细胞对Th2介导的自身免疫发展的影响。获得的五个LEWHg T细胞系可预防（LEW×BN）F1杂种中由氯化汞诱导的Th2介导的自身免疫。这些细胞系除了产生IL-2外，还产生干扰素-γ（IFN-γ）和转化生长因子-β（TGF-β），并且保护作用依赖于TGF-β，因为抗TGF-β处理可消除保护作用。这些结果确定了调节性、产生TGF-β的自身反应性T细胞，它们不同于经典的Th1或Th2，并且可抑制Th1和Th2介导的自身免疫性疾病。
