加拿大安大略省淋病奈瑟菌对喹诺酮类药物敏感性降低的脉冲场凝胶电泳、辅助分型、血清分型及质粒含量分析

Analysis of Neisseria gonorrhoeae in Ontario, Canada, with decreased susceptibility to quinolones by pulsed-field gel electrophoresis, auxotyping, serotyping and plasmid content.

作者信息

Harnett N, Brown S, Riley G, Terro R, Krishnan C, Pauzé M, Yeung K H

机构信息

Central Public Health Laboratory, Ontario Ministry of Health, Toronto, Canada.

出版信息

J Med Microbiol. 1997 May;46(5):383-90. doi: 10.1099/00222615-46-5-383.

DOI:10.1099/00222615-46-5-383

PMID:9152033

Abstract

The incidence of Neisseria gonorrhoeae with reduced susceptibility to quinolones increased from 0.18% (63 of 3285) in 1992 to 0.56% (15 of 2663) in 1993 and 0.62% (46 of 2846) in 1994. In all, 65 of the 67 isolates of Neisseria gonorrhoeae with decreased susceptibility to quinolones were characterised by pulsed-field gel electrophoresis (PFGE), auxotyping, serotyping and plasmid content. The strains were distributed among 14 auxotype/serovar (A/S) classes. Thirty isolates (46.2%) which were penicillin-susceptible with ciprofloxacin MIC90 of 0.12 mg/L and norfloxacin MIC90 of 1.0 mg/L belonged to a single A/S class, OUHL/IA-2. All but two of the 30 isolates had identical PFGE restriction profiles with NheI restriction endonuclease. Fifteen isolates (23.1%) with MICs in the intermediate (or resistant) categories for penicillin and with ciprofloxacin and norfloxacin MIC90 of 0.25 and 4.0 mg/L and (0.5 and 4.0 mg/L) respectively, belonged to A/S class P/IB-1. The 15 isolates showed nine different patterns with NheI and eight patterns with SpeI restriction endonucleases. Two of three beta-lactamase-producing (PPNG) isolates belonged to A/S class P/IB-5 and had a dissimilar PFGE restriction profile with NheI endonuclease; the other isolate belonged to A/S class P/IB-8. The remaining 17 isolates were distributed among 11 A/S classes. Three isolates within the common A/S class NR/IB-1 were subdivided into two types by PFGE as were three isolates belonging to A/S class NR/IB-2. Overall the 65 isolates of N. gonorrhoeae were distributed into 30 NheI and 26 SpeI macrorestriction profiles. All but one isolate harboured the 2.6-MDa cryptic plasmid and 18 isolates carried the 24.5-MDa transferable plasmid. The three PPNG isolates carried the 4.5-MDa Asian beta-lactamase-producing plasmid and a 25.2-MDa conjugative plasmid was found in the two TRNG isolates.

摘要

对喹诺酮类药物敏感性降低的淋病奈瑟菌的发生率从1992年的0.18%（3285例中的63例）增至1993年的0.56%（2663例中的15例）以及1994年的0.62%（2846例中的46例）。总共，对喹诺酮类药物敏感性降低的67株淋病奈瑟菌中的65株通过脉冲场凝胶电泳（PFGE）、辅助分型、血清分型和质粒含量进行了特征分析。这些菌株分布在14个辅助型/血清型（A/S）类别中。30株（46.2%）对青霉素敏感、环丙沙星MIC90为0.12mg/L且诺氟沙星MIC90为1.0mg/L的菌株属于单一的A/S类别，即OUHL/IA - 2。30株中的除两株外的所有菌株用NheI限制性内切酶具有相同的PFGE限制性图谱。15株（23.1%）对青霉素处于中介（或耐药）类别、环丙沙星和诺氟沙星MIC90分别为0.25mg/L和4.0mg/L（以及0.5mg/L和4.0mg/L）的菌株属于A/S类别P/IB - 1。这15株菌株用NheI显示出9种不同模式，用SpeI限制性内切酶显示出8种模式。3株产β - 内酰胺酶（PPNG）的菌株中的2株属于A/S类别P/IB - 5，用NheI内切酶具有不同的PFGE限制性图谱；另一株菌株属于A/S类别P/IB - 8。其余17株菌株分布在11个A/S类别中。常见的A/S类别NR/IB - 1中的3株菌株通过PFGE被细分为两种类型，属于A/S类别NR/IB - 2的3株菌株也是如此。总体而言，65株淋病奈瑟菌被分为30种NheI和26种SpeI宏观限制性图谱。除1株菌株外的所有菌株都携带2.6 - MDa隐蔽质粒，18株菌株携带24.5 - MDa可转移质粒。3株PPNG菌株携带4.5 - MDa亚洲产β - 内酰胺酶质粒，在2株TRNG菌株中发现了一个25.2 - MDa接合性质粒。