Harnett N, Brown S, Riley G, Terro R, Krishnan C, Pauzé M, Yeung K H
Central Public Health Laboratory, Ontario Ministry of Health, Toronto, Canada.
J Med Microbiol. 1997 May;46(5):383-90. doi: 10.1099/00222615-46-5-383.
The incidence of Neisseria gonorrhoeae with reduced susceptibility to quinolones increased from 0.18% (63 of 3285) in 1992 to 0.56% (15 of 2663) in 1993 and 0.62% (46 of 2846) in 1994. In all, 65 of the 67 isolates of Neisseria gonorrhoeae with decreased susceptibility to quinolones were characterised by pulsed-field gel electrophoresis (PFGE), auxotyping, serotyping and plasmid content. The strains were distributed among 14 auxotype/serovar (A/S) classes. Thirty isolates (46.2%) which were penicillin-susceptible with ciprofloxacin MIC90 of 0.12 mg/L and norfloxacin MIC90 of 1.0 mg/L belonged to a single A/S class, OUHL/IA-2. All but two of the 30 isolates had identical PFGE restriction profiles with NheI restriction endonuclease. Fifteen isolates (23.1%) with MICs in the intermediate (or resistant) categories for penicillin and with ciprofloxacin and norfloxacin MIC90 of 0.25 and 4.0 mg/L and (0.5 and 4.0 mg/L) respectively, belonged to A/S class P/IB-1. The 15 isolates showed nine different patterns with NheI and eight patterns with SpeI restriction endonucleases. Two of three beta-lactamase-producing (PPNG) isolates belonged to A/S class P/IB-5 and had a dissimilar PFGE restriction profile with NheI endonuclease; the other isolate belonged to A/S class P/IB-8. The remaining 17 isolates were distributed among 11 A/S classes. Three isolates within the common A/S class NR/IB-1 were subdivided into two types by PFGE as were three isolates belonging to A/S class NR/IB-2. Overall the 65 isolates of N. gonorrhoeae were distributed into 30 NheI and 26 SpeI macrorestriction profiles. All but one isolate harboured the 2.6-MDa cryptic plasmid and 18 isolates carried the 24.5-MDa transferable plasmid. The three PPNG isolates carried the 4.5-MDa Asian beta-lactamase-producing plasmid and a 25.2-MDa conjugative plasmid was found in the two TRNG isolates.
对喹诺酮类药物敏感性降低的淋病奈瑟菌的发生率从1992年的0.18%（3285例中的63例）增至1993年的0.56%（2663例中的15例）以及1994年的0.62%（2846例中的46例）。总共，对喹诺酮类药物敏感性降低的67株淋病奈瑟菌中的65株通过脉冲场凝胶电泳（PFGE）、辅助分型、血清分型和质粒含量进行了特征分析。这些菌株分布在14个辅助型/血清型（A/S）类别中。30株（46.2%）对青霉素敏感、环丙沙星MIC90为0.12mg/L且诺氟沙星MIC90为1.0mg/L的菌株属于单一的A/S类别，即OUHL/IA - 2。30株中的除两株外的所有菌株用NheI限制性内切酶具有相同的PFGE限制性图谱。15株（23.1%）对青霉素处于中介（或耐药）类别、环丙沙星和诺氟沙星MIC90分别为0.25mg/L和4.0mg/L（以及0.5mg/L和4.0mg/L）的菌株属于A/S类别P/IB - 1。这15株菌株用NheI显示出9种不同模式，用SpeI限制性内切酶显示出8种模式。3株产β - 内酰胺酶（PPNG）的菌株中的2株属于A/S类别P/IB - 5，用NheI内切酶具有不同的PFGE限制性图谱；另一株菌株属于A/S类别P/IB - 8。其余17株菌株分布在11个A/S类别中。常见的A/S类别NR/IB - 1中的3株菌株通过PFGE被细分为两种类型，属于A/S类别NR/IB - 2的3株菌株也是如此。总体而言，65株淋病奈瑟菌被分为30种NheI和26种SpeI宏观限制性图谱。除1株菌株外的所有菌株都携带2.6 - MDa隐蔽质粒，18株菌株携带24.5 - MDa可转移质粒。3株PPNG菌株携带4.5 - MDa亚洲产β - 内酰胺酶质粒，在2株TRNG菌株中发现了一个25.2 - MDa接合性质粒。
