Dunnette S L, Gleich G J, Miller R D, Kyle R A
J Immunol. 1977 Nov;119(5):1727-31.
A double antibody radioimmunoassay (RIA) was developed to measure IgD in serum and secretions. One IgD myeloma protein was used as radiolabeled antigen and standard with antiserum to a second IgD myeloma protein. The IgD standard and normal sera yielded parallel inhibition curves in the RIA and inhibition was produced by IgD and not by any of the other immunoglobulins. The assay had a lower limit of sensitivity of 0.01 International Unit (I.U.)/ml and modifications increased the sensitivity to 0.0008 I.U./ml. Measurable IgD levels were found in all 112 normal adult sera assayed (geometric mean 13.0 I.U./ml, arithmetic mean 30.1 I.U./ml, median 14.8 I.U./ml, range 0.10 to 202 I.U./ml, 95% of values between 0.19 and 156 I.U./ml. The distribution of IgD in the 112 normal sera appeared trimodal with modes at approximately 0.25 I.U./ml, 5I.U./ml, and 35 I.U./ml. IgD was measurable in nasal and bronchial washes and human milk, but could not be detected in parotid fluid.
开发了一种双抗体放射免疫测定法（RIA）来测量血清和分泌物中的IgD。使用一种IgD骨髓瘤蛋白作为放射性标记抗原，并与针对另一种IgD骨髓瘤蛋白的抗血清一起作为标准品。在RIA中，IgD标准品和正常血清产生平行抑制曲线，且抑制作用是由IgD产生的，而非其他任何免疫球蛋白。该测定法的灵敏度下限为0.01国际单位（I.U.）/毫升，经过改进后灵敏度提高到0.0008 I.U./毫升。在所检测的112份正常成人血清中均发现了可测量的IgD水平（几何平均值为13.0 I.U./毫升，算术平均值为30.1 I.U./毫升，中位数为14.8 I.U./毫升，范围为0.10至202 I.U./毫升，95%的值在0.19至156 I.U./毫升之间）。112份正常血清中IgD的分布呈三峰型，峰值分别约为0.25 I.U./毫升、5 I.U./毫升和35 I.U./毫升。在鼻腔和支气管冲洗液以及人乳中可检测到IgD，但在腮腺液中未检测到。
