Leslie G A, Teramura G
Int Arch Allergy Appl Immunol. 1977;54(5):451-6.
A simple but sensitive radioimmunoassay method for the quantitation of IgD in sera and body fluids has been developed. Specifically purified chicken anti-delta was covalently coupled to cyanogen bromide activated filter paper discs and the discs were incubated together with the samples to be analyzed. The washed discs were next incubated with 125-I-labeled specifically purified chicken or rabbit anti-human delta-antibodies. IgD on the disc was quantified by reference to a standard curve prepared with purified human IgD. The sensitivity of this assay was approximately 10 ng/ml IgD. IgD was demonstrable in cord sera (18 of 18), hypogammaglobulinemic sera (15 of 15), amniotic fluids (6 of 6), salivas (8 of 10), normal cerebrospinal fluids (12 of 13) and cerebrospinal fluids from patients with subacute sclerosing panencephalitis (5 of 5). The finding of IgD in human secretions raises the interesting possibility that IgD may be a secretory antibody.
已开发出一种用于定量血清和体液中IgD的简单但灵敏的放射免疫测定方法。将特异性纯化的鸡抗δ共价偶联到溴化氰活化的滤纸圆盘上，并将圆盘与待分析的样品一起孵育。接下来，将洗涤后的圆盘与125-I标记的特异性纯化的鸡或兔抗人δ抗体孵育。通过参考用纯化的人IgD制备的标准曲线对圆盘上的IgD进行定量。该测定法的灵敏度约为10 ng/ml IgD。在脐带血清（18例中的18例）、低丙种球蛋白血症血清（15例中的15例）、羊水（6例中的6例）、唾液（10例中的8例）、正常脑脊液（13例中的12例）和亚急性硬化性全脑炎患者的脑脊液（5例中的5例）中均可检测到IgD。在人类分泌物中发现IgD提出了一个有趣的可能性，即IgD可能是一种分泌性抗体。
