Shafritz D A
Gastroenterology. 1979 Dec;77(6):1335-48.
A major avenue of our research has been to develop molecular probes (cDNAs) for studying the pathogenesis of liver disease (i.e., applied molecular pathophysiology). During the last 2-3 yr, we have developed and used molecular hybridization to study regulation of albumin synthesis in normal, protein-calorie deprived, uremic, and cirrhotic rat liver. Our current work is directed toward cloning the albumin gene to permit further analysis of albumin transcriptional and posttranscriptional control. Molecular hybridization, DNA cloning, related techniques can be utilized to study the function of virtually any gene. Incorporation of such advances in basic research into meaningful studies of liver disease is an exciting challenge to modern academic hepatology.
我们研究的一个主要方向是开发用于研究肝脏疾病发病机制（即应用分子病理生理学）的分子探针（cDNA）。在过去的两到三年里，我们已经开发并使用分子杂交技术来研究正常、蛋白质 - 热量缺乏、尿毒症和肝硬化大鼠肝脏中白蛋白合成的调节。我们目前的工作旨在克隆白蛋白基因，以便进一步分析白蛋白转录和转录后调控。分子杂交、DNA克隆及相关技术可用于研究几乎任何基因的功能。将这些基础研究的进展纳入对肝脏疾病有意义的研究中，是现代学术肝脏病学面临的一项令人兴奋的挑战。
